Cat #
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Description
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Literature
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ACA |
COM |
Buy |
ETR4620
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Express-In transfection reagent 0.5ml
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$106
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$157
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for easily moving thousands of open reading frames into your choice of protein expression systems
The Center for Cancer Systems Biology of the Dana-Farber Institute has
created a path-breaking collection of ORF-only clones (full-length
cDNAs with UTRs removed) now with over 12,000 genes1
and with future updates planned. These clones are derived from fully
sequenced Mammalian Gene Collection (MGC) full-length cDNAs and cloned
into a recombinational entry vector.
- Time savings : ORF
clones provide a shortcut to protein expression, allowing you to skip
PCR, cloning into an expression vector, and verifying the ends of the
ORF DNA sequence.
- High fidelity : Each CDS was amplified for only 25 cycles with gene-specific primers and KOD HiFi Polymerase2, greatly minimizing the risk of PCR-induced mutations.
- Ease of transfer: The Gateway®
entry vectors ensure easy transfer into prokaryotic, mammalian, viral,
or insect expression systems. For maximum flexibility, the ORF stop
codon has been removed.
2KOD HiFi Polymerase is supplied by Novagen.
Gateway is a registered trademark of Invitrogen.
Shipping Information:
Individual clones:
Clones are provided as a live culture in a 2-ml tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2–3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.
Clone Collections:
Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.
