The Center for Cancer Systems Biology of the Dana-Farber Institute has
created a path-breaking collection of ORF-only clones (full-length
cDNAs with UTRs removed) now with over 12,000 genes1,2,3
and with future updates planned. These clones are derived from fully
sequenced Mammalian Gene Collection (MGC) full-length cDNAs and cloned
into a recombinational entry vector.
- Time savings : ORF
clones provide a shortcut to protein expression, allowing you to skip
PCR, cloning into an expression vector, and verifying the ends of the
ORF DNA sequence.
- High fidelity : Each CDS was amplified for only 25 cycles with gene-specific primers and KOD HiFi Polymerase4, greatly minimizing the risk of PCR-induced mutations.
- Ease of transfer: The Gateway®
entry vectors ensure easy transfer into prokaryotic, mammalian, viral,
or insect expression systems. For maximum flexibility, the ORF stop
codon has been removed.
4KOD HiFi Polymerase is supplied by Novagen.
Gateway is a registered trademark of Invitrogen.
1. Rual, JR et al (2004). Human ORFeome Version 1.1: a platform for reverse proteomics. Genome Research14, 2128–2135
2. Lamesch et al (2007) hORFeome 3.1: a resource of human open reading frames covering over 10,000 human genes. Genomics89:307-15.
3. Rual et al (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature437:1173-8, 2005.
