The Precision LentiORF collection is a library of expression-ready, lentiviral open reading frames (ORFs) generated using gene content from the sequence-validated ORFeome Collaboration Collection. These ready-to-use ORFs are high-quality, versatile and guaranteed to express. Available formats include ready-to-use lentiviral particles and starter kits for guaranteed* expression results.
- Fully sequenced ORFs for increased confidence in gene expression results
- Dual marker ORF cassette provides two ways to optimize transduction
- Nuclear localized TurboGFP for expression tracking
- Blasticidin resistance for selection
- Lentiviral transduction extends gene expression to a broad range of cell types including primary and non-dividing cells
- Guaranteed expression
Please note that the Precision LentiORF expression vectors are not compatible with third generation packaging systems such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with these vectors.
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Nuclear localized TurboGFP expressed from cells transduced with Precision LentiORF viral particles. |
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Immunocytochemistry of HEK293T cells showing cellular morphology of cytoplasm (Anti-tubulin - red) and nucleus (DAPI - blue). A, Untransduced cells undergoing typical cell division. B, Cells transduced with Precision LentiORFs expressing PLK1 have undergone endoreduplication without mitosis. Increased PLK1 expression and this phenotype are common to many types of cancer. |
The Precision LentiORF library can be purchased in the following formats:
Individual clones - Glycerol stock or high titer (10^8 TU/ml) virus
LentiORF starter kits - Transfection or transduction kits
Kits include your choice of two LentiORFs, control and delivery reagent (Express-In transfection reagent or Trans-Lentiviral packaging kit)
*When used as part of a LentiORF starter kit according to kit instructions
Finding your Lenti-ORF of interest:
Enter gene symbol(s), GenBank accession number(s), clone IDs, unigene clusters or catalog numbers into the gene search (
) Results will be returned in a tabbed format. Click on the ORFs tab to find ORFs expressing your gene of interest. Order directly online. Constructs are shipped in 48 hours.
Alternatively you can send your gene symbols or accession numbers to us at info@openbiosystems.com and technical representatives will perform your search for you.
This product is covered by the following limited use licenses:
TurboGFP and
TurboRFP are covered by a
Limited Use License from Evrogen.
