Figure 1. Putative full-length clone failure rate in the MGC pipeline. Cumulative numbers for putative and verified full-length clones over the history of the MGC through August 28, 2005 (from the MGC weekly status report).
Figure 2. Splice variants may have identical terminal exons and similar overall length. Two splice variant cDNAs for the aurora kinase C gene (AURKC) retain the same terminal exons (1 and 7), but include either one of two internal exons (4 or 5). The two isoforms were not distinguishable by agarose gel electrophoresis.1
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EST and putative full-length clones may not be full-length, because of incomplete ORFs, chimeric sequences, or frameshifts (Figure 1).
- The entire sequence is needed for correct identification of alternative splice isoform cDNAs that typically code for alternative proteins with alternative functions (Figure 2).
Baross A et al systematic recovery recovery and analysis of full-ORF human cDNA clones. Genome research 14, 2083 - 2092.
