Jodi Parrish et al¹ from the Center for Molecular Medicine and Genetics, and Department of Biochemistry and Molecular Biology, Wayne State University have cloned 1,619 ORFs from Campylobacter jejuni into E. coli using in vivo recombination. The ORFs that were chosen include 1654 ORFs that were predicted by the Sanger Institute² , together with 31 additional ORFs predicted by TIGR (http://www.tigr.org/tigr-scripts/CMR2/CMRHomePage.spl). The availability of these ORFs circumvents a common rate-limiting step in proteomics, the generation of an appropriate predicated ORF clone set for use in proteome-scale micro-array analysis. Their use in micro-array studies to profile gene expression patterns will be invaluable to the research community and will provide a quick and inexpensive way to investigate genes and proteins and understand their function and regulation.

E. coli SURE clones containing the C. jejuni ORFs in the vector pTLJ03 are available from Open Biosystems. pTLJ03 is an expression vector that generates N-terminal GST-His-tagged fusion proteins from the C. jejuni ORF set. Plasmid maps, details, and sequences are either available below or at http://proteome.wayne.edu. Recombination sites were verified by sequencing, and most clones have been 5’ sequenced. The ORFs range from 45 – 4,020bp in length.

Note: pTLJ03 is not a high-copy number plasmid. The C. jejuni ORF clones are cultured in LB with 100ug/ml Ampicillin.

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