for systematic functional analysis of the E. coli genome
Developed as a collaboration between the Institute for Advanced Biosciences, Keio University (Japan), Nara Institute of Science and Technology (Japan), and Purdue University, the E. coli Keio Knockout Collection contains deletion strains for all nonessential genes in E. coli.
- There are two independent deletion strains for each of 3985 genes, yielding a total of 7970 strains
- Each deleted gene is replaced with a kanamycin resistance cassette that can be excised by FLP recombination
- Strain background: E. coli K-12 BW25113
Individual strains are available from the E. coli Genetic Stock Center at Yale University.
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| Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products. |
The E. coli Keio Knockout Collection is provided as glycerol stocks in microtiter plates. These will ship on dry ice for overnight delivery and should be stored at –80°C immediately upon arrival. Please contact our customer
service department for estimated shipping times.
Baba, T et al (2006). Construction of Escherichia coli K12 in-frame, single-gene knockout mutants: the Keio collection. Molecular Systems Biology doi:10.1038/msb4100050
Datsenko KA and BL Wanner (2000). One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. PNAS97, 6640–6645
