Created by a consortium of laboratories¹, the BrucellaORF Collection is comprised of 3091 ORFs that have been cloned into a Gateway® entry vector and are ready to be transferred into Gateway destination vectors for protein expression and functional analysis (Figure 1). For example, protein-protein interactions can be studied by transferring the collection to bait and prey plasmids for yeast two-hybrid screening or to a destination construct that expresses each ORF with an affinity, epitope, or fluorescent tag.

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