an Open Labs Product for gene functional analysis of an intracellular pathogen
Created by a consortium of laboratories1, the BrucellaORF Collection is comprised of 3091 ORFs that have been cloned into a Gateway®
entry vector and are ready to be transferred into Gateway destination
vectors for protein expression and functional analysis (Figure 1). For
example, protein-protein interactions can be studied by transferring
the collection to bait and prey plasmids for yeast two-hybrid screening
or to a destination construct that expresses each ORF with an affinity,
epitope, or fluorescent tag.
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| We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control. Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. Thermo Fisher Scientific is not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting. |
Gateway is a trademark of Invitrogen Corporation.Brucella ORF clones are available as glycerol stocks. Individual clones are shipped in
microtubes at room temperature, while the entire collection is shipped
in 96-well plates on dry ice. Microtubes and plates should be stored at
–80ºC.
References:
1Dricot A et al (2004). Generation of the Brucella melitensis ORFeome Version 1.1. Genome Research14, 2201–2206
