an Open Labs Product for gene functional analysis of an intracellular pathogen
Created by a consortium of laboratories1, the BrucellaORF Collection is comprised of 3091 ORFs that have been cloned into a Gateway®
entry vector and are ready to be transferred into Gateway destination
vectors for protein expression and functional analysis (Figure 1). For
example, protein-protein interactions can be studied by transferring
the collection to bait and prey plasmids for yeast two-hybrid screening
or to a destination construct that expresses each ORF with an affinity,
epitope, or fluorescent tag.
|
 |
| Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products. |
Gateway is a trademark of Invitrogen Corporation.Brucella ORF clones are available as glycerol stocks. Individual clones are shipped in
microtubes at room temperature, while the entire collection is shipped
in 96-well plates on dry ice. Microtubes and plates should be stored at
–80ºC.
References:
1Dricot A et al (2004). Generation of the Brucella melitensis ORFeome Version 1.1. Genome Research14, 2201–2206
