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Home |  Gene Expression | Non-Mammalian Resources | Bacteria | C. jejuni ORFs
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OCJ1761
 ORF: C. jejuni ORF Clone   $75 $75 Click to show search field
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OCJ1762 ORF: C. jejuni ORF Collection   $2520 $5670
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C. jejuni ORFs
Jodi Parrish et al1 from the Center for Molecular Medicine and Genetics, and Department of Biochemistry and Molecular Biology, Wayne State University have cloned 1,619 ORFs from Campylobacter jejuni into E. coli using in vivo recombination. The ORFs that were chosen include 1654 ORFs that were predicted by the Sanger Institute2 , together with 31 additional ORFs predicted by TIGR (http://www.tigr.org/tigr-scripts/CMR2/CMRHomePage.spl). The availability of these ORFs circumvents a common rate-limiting step in proteomics, the generation of an appropriate predicated ORF clone set for use in proteome-scale micro-array analysis. Their use in micro-array studies to profile gene expression patterns will be invaluable to the research community and will provide a quick and inexpensive way to investigate genes and proteins and understand their function and regulation.

E. coli SURE clones containing the C. jejuni ORFs in the vector pTLJ03 are available from Open Biosystems. pTLJ03 is an expression vector that generates N-terminal GST-His-tagged fusion proteins from the C. jejuni ORF set. Plasmid maps, details, and sequences are either available below or at http://proteome.wayne.edu. Recombination sites were verified by sequencing, and most clones have been 5’ sequenced. The ORFs range from 45 – 4,020bp in length.

Note: pTLJ03 is not a high-copy number plasmid. The C. jejuni ORF clones are cultured in LB with 100ug/ml Ampicillin.

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Shipping Information:
Individual clones:
Clones are provided as a live culture in a 2ml tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2-3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.

Bulk Orders:
Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

Entire Collection:
Orders for the entire C. jejuni collection are provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for estimated shipping time on orders for the entire collection.
 
References:
1 Parrish J, Limjindaporn T, Hines JA, Liu J, Liu G, and Finley RL, Jr. High-throughput cloning of Campylobacter jejuni ORFs by in vivo recombination in Escherichia coli,J. Proteome Research, in press (2004).
2 Parkhill J, Wren BW, Mungall K, Ketley JM, Churcher C, Basham D, Chillingworth T, Davies RM, Feltwell T, Holroyd S, Jagels K, Karlyshev AV, Moule S, Pallen MJ, Penn CW, Quail MA, Rajandream MA, Rutherford KM, van Vliet AH, Whitehead S, Barrell BG. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature. 2000 Feb 10;403(6770):665-8.
 

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