an Open Labs Product for investigating gene regulation
Researchers at the Weizmann Institute of Science have produced a collection of E. coli
strains that enables monitoring of gene expression at high temporal
resolution in living cells. Each of the reporter strains has a bright,
fast-folding green fluorescent protein (GFP) fused to a full-length
copy of an E. coli promoter in a low-copy plasmid.
This collection includes more than 1900 promoters (out of 2500 in the entire genome) for E. coli
K12 strain MG1655 and enables measurement of gene expression at a
resolution of minutes with high accuracy and reproducibility.
Performing experiments in a multi-well fluorimeter using FACS or
time-lapse fluorescence microscopy has the necessary sensitivity to
measure gene expression in individual cells.
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| Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products. |
Individual clones:
Clones are provided as a live culture in a
2-ml tube. Each tube contains LB medium supplemented with 8% glycerol
and kanamycin. Within 2–3 business days of receiving your order we will
ship your clone at room temperature via express delivery. Store the
stock clone at –80°C.
Bulk Orders:
Orders of 50 clones
or greater and stock plates will be provided in microtiter plates.
These will ship on dry-ice via overnight delivery and should be stored
at –80°C immediately upon receipt. Please contact our customer service
department for a quote and estimated shipping time on bulk orders.
Entire Collection:
Orders
for the entire E. coli Promoter Collection are provided in microtiter
plates. These will ship on dry-ice via overnight delivery and should be
stored at –80°C immediately upon receipt. Please contact our customer
service department for estimated shipping time on orders for the entire
collection.
References:
Kalir S et al (2001). Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science 292, 2080–2083
Ronen M et al (2002). Assigning numbers to the arrows: parameterizing a gene regulation network by using accurate expression kinetics. Proc. Natl. Acad. Sci. USA 99, 10555–10560
Setty et al (2003). Proc. Natl. Acad. Sci. USA 100, 7702–7707
Zaslaver A et al (2004). Just-in-time transcription program in metabolic pathways. Nature Genetics 36, 486–491
Zaslaver A, Anat Bren, Michal Ronen, Shalev Itzkovitz, Ilya Kikoin, Seagull Shavit, Wolfram Liebermeister, Michael G Surette & Uri Alon (2006). A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nature Methods3, 623–628.
