Singapore's Institute for Molecular and Cell Biology (IMCB) has isolated over 15,000 unique genetic markers from the zebrafish (Danio rerio). A total of 15,590 unique zebrafish EST clusters from two cDNA libraries (primary and normalized) have been identified. This EST set is complementary to the existing ESTs in the public database and will be invaluable in assisting in the annotation of genes based on the upcoming zebrafish genome sequence. Their use in microarray studies to profile gene expression patterns, especially in embryogenesis, will provide a quick and inexpensive way to discover new genes and understand their regulation in the body.
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