The ceORFs were generated by amplification from full length cDNA libraries and cloning into the Gateway®² recombinational cloning system (pDONR201). ceORF clones can be subcloned into any expression vector, using traditional restriction enzyme and ligase cloning methods. Alternatively, the recombinational cloning system allows the target gene to be rapidly transferred in-frame into a variety of expression vectors. Release 1.1 clones represent a pool of cloned products, thus multiple splice variants are preserved in each pool. The ceORF clones in Release 1.1 have been end sequence tagged³ (OST) to confirm the identity of the gene and the presence of at least one splicing event. Subsequent releases will be occurring as further refinements of the ceORF clones occur. This on-going process includes in-depth characterization by interaction mapping, RNAi screening and full insert sequencing. Watch our website for further details and links to these interesting projects.
ceORFs will be available in two different formats: glycerol stocks and spotted onto IsoCode® cards. The ceORFs spotted onto IsoCode® cards give you the freedom to store the clones at ambient temperature and use them directly in PCR, transformation and sequencing. Please email or call 888-412-2225 our Customer Service representatives if you would like the IsoCode® card format.

Benefits:

• Coverage of greater than 60% of the C. elegans genome
• Pools of cloned amplimers cover splice variants for a specific gene
• Continued characterization efforts expands the annotation
• Optional delivery on IsoCode® cards enables you to store the clones at ambient temperature and provides a means for direct usage in PCR, transformation and sequencing