Home |  Gene Expression | Non-Mammalian Resources | Worm | C. elegans ORF-RNAi Collection
Purchasing Information
Cat # Description Literature ACA COM Buy
RCE1182
C elegans RNAi V1.1 Clone   $100 $100 Click to show search field
Search:
(only clones from RCE1182)
RCE1181 C elegans RNAi Collection V1.1   $10000 call
Search:
(only clones from RCE1181)
search:Search add to cart:Cart product kit: Kit product insert:Literature datafile:Data File

C. elegans ORF-RNAi Collection
The C. elegans ORF-RNAi Collection1 includes clones containing full open reading frames for over 11,000 genes, ready for your RNAi screens! These clones are derived from the C. elegans ORFeome Library v1.1.  The C. elegans ORF-RNAi Library provides you comprehensive coverage for RNAi screening.
  • Full Coding Regions: From Start-to-Stop, the entire open reading frame is cloned into an RNAi feeding vector and compatible host.
  •  Feeding Ready: The cloned ORFs have been transformed into the RNAi feeding bacterial strain HT115(DE3)2 and the feeding vector, PL4440.
  • RNAi Versatility: Plasmid preps of the ORF-RNAi clones can be used as templates for in vitro dsRNA synthesis for soaking or injection

What is Open Labs?
Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products.

Shipping Information:
Individual clones:
Clones are provided as a live culture in a 2ml tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. We will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.

Bulk Orders:
Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

Entire Collection:
Orders for the entire C. elegans ORF-RNAi collection are provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for estimated shipping time on orders for the entire collection.

References:
1Towards Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based RNAi Library. Jean-François Rual, Julian Ceron, John Koreth, Tong Hao, Anne-Sophie Nicot, Tomoko Hirozane-Kishikawa, Jean Vandenhaute, Stuart H. Orkin, David E. Hill, Sander van den Heuvel, and Marc Vidal Genome Research In Press
2Timmons, L. and Fire, A. 1998. Specific interference by ingested dsRNA. Nature395: 854.
 
WEB SITE REFERENCES
http://www.wormbase.org ; Source for C. elegans genome and RNAi data.
http://worfdb.dfci.harvard.edu ; Source for C. elegans ORFeome data.
http://nematoda.bio.nyu.edu ; Source for C. elegans RNAi data.

© 2005-2007 Open Biosystems All Rights Reserved