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Home |  Gene Expression | Non-Mammalian Resources | Worm | C. elegans ORF-RNAi Collection
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Cat # Description Literature ACA COM Buy
RCE1182
 C. elegans RNAi V1.1 Clone   $108.00* $108.00* Click to show search field
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RCE1181 C. elegans RNAi Collection V1.1   call call
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C. elegans ORF-RNAi Collection
The C. elegans ORF-RNAi Collection1 includes clones containing full open reading frames for over 11,000 genes, ready for your RNAi screens! These clones are derived from the C. elegans ORFeome Library v1.1.  The C. elegans ORF-RNAi Library provides you comprehensive coverage for RNAi screening.
  • Full Coding Regions: From Start-to-Stop, the entire open reading frame is cloned into an RNAi feeding vector and compatible host.
  •  Feeding Ready: The cloned ORFs have been transformed into the RNAi feeding bacterial strain HT115(DE3)2 and the feeding vector, PL4440.
  • RNAi Versatility: Plasmid preps of the ORF-RNAi clones can be used as templates for in vitro dsRNA synthesis for soaking or injection

Please Note:
We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control. Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. Thermo Fisher Scientific is not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.

Shipping Information:
Individual clones:
Clones are provided as a live culture in a 2ml tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. We will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.

Bulk Orders:
Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

Entire Collection:
Orders for the entire C. elegans ORF-RNAi collection are provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for estimated shipping time on orders for the entire collection.

References:
1Towards Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based RNAi Library. Jean-François Rual, Julian Ceron, John Koreth, Tong Hao, Anne-Sophie Nicot, Tomoko Hirozane-Kishikawa, Jean Vandenhaute, Stuart H. Orkin, David E. Hill, Sander van den Heuvel, and Marc Vidal Genome Research In Press
2Timmons, L. and Fire, A. 1998. Specific interference by ingested dsRNA. Nature395: 854.
 
WEB SITE REFERENCES
http://www.wormbase.org ; Source for C. elegans genome and RNAi data.
http://worfdb.dfci.harvard.edu ; Source for C. elegans ORFeome data.
http://nematoda.bio.nyu.edu ; Source for C. elegans RNAi data.