Cat #
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Description
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Literature
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ACA |
COM |
Buy |
OCE4518
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C. elegans ORF Collection (Releases 1+2)
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call
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call
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RCE1181
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C elegans RNAi Collection V1.1
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$10000
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call
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PCE1181
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C elegans Promoters Library
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$7100
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call
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for yeast one- and two-hybrid assays
Yeast one-hybrid (Y1H) assays are a gene-centered alternative to the more traditional ChIP on Chip approach to the mapping of transcription factor regulatory networks. In contrast to ChIP on Chip, Y1H assays work equally well for rare or low-abundance transcription factors and do not require the development of antibodies against each transcription factor. Moreover, because the connectivity of promoters appears to be greater than the connectivity of transcription factors (Deplancke et al, 2006), a gene-centered approach will uncover more interactions quickly. The C. elegansTranscription Factor Collection consists of 755 C. elegans transcription factor ORFs fused with the GAL4p activation domain and cloned into the yeast prey vector pDESTAD.|
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| Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products. |
Shipping Information:
Individual clones and strains are shipped at room temperature while collections are shipped on dry ice. All should be stored at –80° C.
References:
Deplancke B et al (2004). A Gateway-compatible yeast one-hybrid system. Genome Research 14, 2093–2101
Deplancke B et al (2006). A gene-centered C. elegans protein-DNA interaction network. Cell 125, 1193–1205
Vermeirssen V et al (2007). A C. elegans transcription factor array and Steiner Triple System-based smart pools: high-performance tools for transcription regulatory network mapping. Nature Methods 4, 659–664
Walhout AJM (2006). Unravelling transcription regulatory networks by protein-DNA and protein-protein interaction mapping. Genome Research16, 1445–1454.
