a novel enzyme with unusual properties
Rainbow DNA Polymerase is a recombinant family B DNA polymerase cloned from the hyperthermophilic marine archaeon Thermococcus sp.
OGL-20P, isolated from the Rainbow hydrothermal vent field in the
Atlantic Ocean. It is a robust, multi-purpose enzyme that possesses a
proofreading 3’-5’ exonuclease with high fidelity and can be used in
all applications involving primer extension. More thermostable than Taq
polymerase (3 x longer half life at 95°C), Rainbow DNA Polymerase’s
high extension rate and processivity generate superior yields in short
reaction times using a wide range of template types and purity levels.
DNA products are blunt ended.
Shipped on dry ice and stored at –20°C.
References:
1 Rainbow DNA
Polymerase is manufactured by ExtremoZyme Inc., licensed by the
University of Alabama at Huntsville (Huntsville, AL), and distributed
by Open Biosystems, Inc.
2
One unit is defined as the amount of enzyme that will incorporate 10
nmol of dNTP in 30 minutes at 74°C in the presence of Rainbow reaction
buffer, 0.2 mM each dNTP, and 16.6 ng/µL primed M13mp18 substrate.
