Reduce mRNA levels of essential yeast genes by four- to ten-fold with the Decreased Abundance by mRNA Perturbation (DAmP) Yeast Library. Dr. Jonathan Weissman’s lab at the University of California, San Francisco has created a library of essential gene hypomorphic alleles whereby the 3’ untranslated region (UTR) of each essential gene present in the collection was disrupted with an antibiotic resistance cassette. The library is available in either haploid or heterozygous diploid formats.
Possible applications:
- Explore interactions of essential genes
- Sensitive detection of growth rate changes
- High resolution functional analysis of the yeast genome
*Please note that the creators of this resource list the number of genes they believe to be essential in each collection in the product description. DAmP strains were created for additional genes and included in the collections. Therefore the number of strains included in each collection is greater than the number listed in the product description.|
| We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control. Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. Thermo Fisher Scientific is not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting. |
