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Purchasing Information
Cat # Description Literature ACA COM Buy
YSC5050
Yeast DAmp Library for 970 essential genes - diploid   $1,155.00* $1,735.00*
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YSC5090 Yeast DAmP Library for 842 essential genes - haploid   $1,000.00* $1,500.00*
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(only clones from YSC5090)
YSC5093
Yeast DAmP individual strain - diploid   $55.00* $55.00* Click to show search field
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YSC5094 Yeast DAmP individual strain - haploid   $55.00* $55.00* Click to show search field
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(only clones from YSC5094)
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DAmP Yeast Library
Decreased expression of essential genes through mRNA perturbation

Reduce mRNA levels of essential yeast genes by four- to ten-fold with the Decreased Abundance by mRNA Perturbation (DAmP) Yeast Library. Dr. Jonathan Weissman’s lab at the University of California, San Francisco has created a library of essential gene hypomorphic alleles whereby the 3’ untranslated region (UTR) of each essential gene present in the collection was disrupted with an antibiotic resistance cassette. The library is available in either haploid or heterozygous diploid formats.

Possible applications:

  • Explore interactions of essential genes
  • Sensitive detection of growth rate changes
  • High resolution functional analysis of the yeast genome
*Please note that the creators of this resource list the number of genes they believe to be essential in each collection in the product description.  DAmP strains were created for additional genes and included in the collections.  Therefore the number of strains included in each collection is greater than the number listed in the product description.

What is Open Labs?
Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products.
Shipping Information:
Individual clones: Within 2–3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C. Clones are provided as a live culture in a 2-ml tube. Each tube contains:
  • Haploid DAmP strain: YEPD + G418 (200ug/ml) + 15% glycerol.
  • Diploid DAmP strain: SD-URA + G418 (200ug/ml) + 15% glycerol.
High Volume Orders: Orders of greater than 50 clones will be provided in microtiter plates. These will ship on dry ice via overnight delivery and should be stored at –80°C immediately upon receipt.
Complete Collection:
Orders for the entire collection are provided in 96-well microtiter plates. These will ship on dry ice via overnight delivery and should be stored at –80°C immediately upon receipt.

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