for inducible overexpression of yeast ORFs
The Andrews lab at the University of Toronto has developed a collection of more than 5000 yeast strains that each overexpresses a different yeast open reading frame (ORF) when induced with galactose. More than 80% of the S. cerevisiae genome is represented. ORFs are plasmid-encoded, tagged at the N-terminus with GST, and expressed under control of the GAL1/10 promoter (Zhu et al, 2001). This collection is in a BY4741 (MATa) background and has been used for genome-scale overexpression screening (Sopko et al, 2006).|
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Shipping Information:
Yeast strains are shipped in CM broth with glucose –uracil (Teknova cat # C7141) with 15% glycerol (added after growth of strains). Individual strains are shipped in 2-ml tubes at room temperature; the entire collection is shipped in 96-well plates on dry ice. All strains should be stored at –80°C.
References:
Sopko et al (2006). Mapping pathways and phenotypes by systematic gene overexpression. Molecular Cell 21, 319–330.
Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R., Bidlingmaier, S., Houfek, T., Mitchell, T., Miller, P., Dean, R.A., Gerstein, M. and Snyder, M. 2001. Global analysis of protein activities using proteome chips. Science 293: 2101–2105.
