This collection contains the S. cerevisiae genome in an E. coli-yeast 2-micron LEU2 shuttle vector, designed by Dr. Greg Prelich of the Albert Einstein College of Medicine. These 1,588 plasmid clones represent a unique and virtually complete overlapping clone collection of the entire S. cervisiae genome. Plasmid inserts average ~10 kb, with genes expressed untagged from their endogenous promoters. This clone collection constitutes a “minimal functional pathway” through the yeast genome and represents ~97% of the length of the yeast genome at the physical level, and ~95% of the genome at the functional level. This collection was designed primarily for systematic overexpression screens.

Available in two formats:

Pooled Assay Ready DNA

• Entire genome in one tube
• Sufficient DNA provided for 2-3 screens
• No need for thousands of plasmid preps

Glycerol Stock Arrayed Library

• Each clone has been mapped to the yeast genome
• Create chromosome or pathway specific pools
• Endless supply of DNA

More information and protocols can be found on Dr. Prelich's website: http://steelhead.aecom.yu.edu/SystematicLibrary.html

What is Open Labs?

Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products.