- Generate HA epitope tagged proteins for
immunolocalization or immunoprecipitation using antibodies against HA.
- A potential source of conditional alleles and
hypomorphic mutants (generated by transposon insertion of 3X HA Tag).
- Find strains and insertion sites of the HA epitope tag via our user friendly on-line query.
The HA-Tagged Yeast Collection contains over 2,400 yeast mutagenized
strains each producing a single protein with an inserted triple
hemagglutinin epitope tag (3xHA)* . HA tags are useful for a variety of
functional studies including immunolocalization, immunoprecipitation
and analysis of binding sites using immunodetection. Insertion via
mini- transposon (mTn) mutagenesis allows for tagging within the coding
region of the protein in a non-biased fashion thus resulting in the
tagging of both annotated and previously unidentified ORFs1.
The HA insertion potentially provides several means by which yeast
protein function may be studied. HA-tagged yeast proteins have been
used effectively in large-scale studies of protein localization;
full-length HA tagged proteins can be localized to the appropriate
cellular region where they can be detected using an antibody to the HA
epitope2. Native amounts of protein can easily be purified from
individual yeast strains using immunoprecipitation with a commercially
available HA antibody. Also, HA insertion may potentially generate
conditional alleles and hypomorphic mutants that exhibit partial gene
function of particular importance in the analysis of essential genes. |
