Open Biosystems - Yeast Insertional Mutant Strains

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Home | Gene Expression | Yeast | Yeast Insertional Mutant Strai

Purchasing Information
Cat #	Description	Literature	ACA	COM	Buy
YSC1073	Yeast Mini Transponson Clone		$50	$50
Search: (only clones from YSC1073)
YSC1074	Yeast Mini Transponson Collection		$1995	$2610
Search: (only clones from YSC1074)
YSC1202	Yeast mTn plasmid		$50	$50
Search: (only clones from YSC1202)
YSC1201	Yeast mTn plasmid collection		$1995	$2609
Search: (only clones from YSC1201)
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Yeast Insertional Mutant Strains

The Yeast Insertional Mutant collection contains over 3,600 mutagenized yeast strains providing a set of gene disruption mutants for phenotypic analysis^*. The mutagenized strains were developed via transposon insertional mutagenesis. The mini- transposons (mTn) are initially introduced into a plasmid library of yeast genomic DNA followed by transformation of the disruption alleles into a heterozygous diploid strain of yeast. Once introduced into the yeast the transposons integrate into the corresponding genomic locus by homologous recombination. The insertion of the transposon into the open reading frame (ORF) of the gene typically disrupts gene function, or insertion upstream or downstream of the ORF may result in mis-expression of the gene.

The 6kb mTn contains the reporter gene lacZ, flanked by lox sites, and a 3xHA tag. The lacZ gene is lacking both its promoter and start codon, thus, the expression is dependent on the transposon being inserted in-frame into a gene and subsequently transcribed and translated. Using the site-specific DNA recombinase, Cre, the 6kb insert can be reduced to a 93-codon read-through element containing the 3X HA-tag (see HA-Tagged Yeast Collection).The mTn technology has been used for large-scale functional analysis of the yeast genome. Access to the resulting gene data, protocols, and publications is available at the Yale Genome Analysis Center website at http:// ygac.med.yale.edu/triples/triples.htm. Information on the insertion site, ORFs affected, and strains available from Open Biosystems can be obtained by querying on our website by ORF ID. Background strain = Y800 (diploid).

^* In most cases, the insertion disrupts gene function however some insertions may not result in gene disruptions. Additionally, individual researchers should confirm that any phenotype observed is actually linked to the indicated insertion.

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References:

P. Ross-Macdonald, et al., Nature402, 413 (1999).

Kumar, A. et al., Methods Enzymol. 328. 550-574 (2000).

Kumar, A., Cheung, K.-H., Ross-Macdonald, P., Coelho, P.S.R., Miller, P., and Snyder, M. Nucleic Acids Res. 28, 81 (2000).

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