for plasmid-based expression and purification for over 4900 genes
Developed by Eric Phizicky (University of Rochester) and Mike Snyder (Yale University)*, the Yeast ORF Collection enables robust protein expression and purification for over 4900 S. cerevisiae genes. Each plasmid construct is available in yeast or E. coli format as a glycerol stock. - Open reading frame (ORF) clones provide a shortcut to protein expression, allowing you to skip PCR, cloning into an expression vector, and verifying the DNA sequence for each construct.
- All yeast-format constructs have been verified by western blotting to express protein of the correct length.
- The optional E. coli format enables easy conversion to alternative yeast strains.
- The versatile 19-kDa tandem fusion tag enables a range of detection and affinity purification strategies. In addition to Protein A and 6xHis domains, it contains a hemagglutination (HA) domain for which we offer an Anti-HA Antibody.
Because the Yeast ORF Collection is designed for protein overexpression, all ORFs are plasmid encoded and under control of the GAL1 promoter. For accurate profiling of the yeast proteome, we offer the Yeast TAP-Tagged Collection in which each fusion-tagged ORF is under control of the endogenous promoter at its original chromosomal location.
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| Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products. |
Shipping Information:
Individual clones: Within 2–3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C. Clones are provided as a live culture in a 2-ml tube. Each tube contains:- E. coli: LB media supplemented with ampicillin and 8% glycerol
- Yeast: SD-URA medium supplemented with 15% glycerol.
High Volume Orders: Orders of greater than 50 clones will be provided in microtiter plates. These will ship on dry ice via overnight delivery and should be stored at –80°C immediately upon receipt. Complete Collection: Orders for the entire Yeast ORF Collection are provided in 96-well microtiter plates. These will ship on dry ice via overnight delivery and should be stored at –80°C immediately upon receipt.
References:
*Gelperin DM, White MA, Wilkinson ML, Kon Y, Kung LA, Wise KJ, Lopez-Hoyo N, Jiang L, Piccirillo S, Yu H, Gerstein M, Dumont ME, Phizicky, EM, Snyder M, Grayhack EJ (2005). Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes and Development19, 2816–2826.
