Chat
Recent Searches
Part of Thermo Fisher Scientific  
 
 
 
Home |  Gene Expression | Yeast | Yeast TAP-Tagged Collection
Figure 1
 

Figure 2
 

Figure 3
 
Purchasing Information
Cat # Description Literature ACA COM Buy
YSC1177
 TAP Tagged ORF Collection.   $3,860.00* call
Search:
(only clones from YSC1177)
YSC1178 TAP Tagged strain (endogenous promoter)   $55.00* $55.00* Click to show search field
Search:
(only clones from YSC1178)
YSC1247
 Yeast TAP-Fusion Membrane Protein Subset   $830.00* $2,490.00*
Search:
(only clones from YSC1247)
CAB1001 50ug Rabbit Anti-TAP Antibody   $235.00* $235.00*
Search:
(only clones from CAB1001)
search:Search add to cart:Cart product kit: Kit product insert:Literature datafile:Data File

Yeast TAP-Tagged Collection
for profiling the yeast proteome
To facilitate global protein analyses, Dr. Erin O'Shea and Dr. Jonathan Weissman (UCSF) have created a library where each ORF is tagged with a high-affinity epitope and expressed from its natural chromosomal location. Through immunodetection of the common tag, a resource now exists that provides a census of proteins expressed during log-phase growth and quantifies their absolute levels. Characterization of this library revealed that ~80% of the proteome is expressed during normal growth conditions. The abundance of proteins ranged from fewer than 50 to more than 106 molecules/cell with many, including essential proteins and most transcription factors, having levels not readily detectable by other proteomic techniques, nor predictable by mRNA levels or codon bias measurements (Figures 1 & 2)1.

The Yeast-TAP-Fusion Library allows the purification and selection of the entire yeast proteome and associated components using two simple affinity selection steps in tandem, enabling the development of a range of high-throughput functional assays. A collection of ORF specific oligonucleotide primers were synthesized. Each primer pair possesses shared 3' ends that allow for PCR amplification of a common insertion cassette, as well as gene-specific 5' ends that allow for the precise introduction, through homologous recombination, of the amplified insertion cassettes as a perfect in-frame fusion at the C-terminal end of the coding region of each gene (Figure 3).

 The C-terminal TAP insertion cassette contains the coding region for a modified version of the TAP (Tandem Affinity Purification) tag, which consists of a calmodulin binding peptide, a TEV cleavage site and two IgG binding domains of Staphylococcus aureus protein A, as well as a selectable marker. Background strain = MatA (BY4741). The Anti-TAP antibody is highly specific for the TAP.

What is Open Labs?
Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products.
Shipping Information:
Individual strains are provided in tubes containing glycerol stock cultures in YPD plus 15% glycerol. While the scTAP strains are supplied in YPD media, SD complete or SD -HIS media can also be used. Individual strains from this library can be queried and purchased through our clone query.

The TAP Library is provided in 96-well microtiter plates containing frozen glycerol stock cultures in YPD plus 15% glycerol. While the scTAP strains are supplied in YPD media, SD complete or SD -HIS media can also be used. Strains are arranged in plates by protein expression size from largest to smallest, with plate designation GS1 representing high expression and GS5 representing low expression. In addition a membrane bound protein subset has been arrayed by Open Biosystems in separate plates for your convenience. Refer to CD shipped with the collection for expression category designation.
References:

1Ghaemmaghami, S., Huh, W., Bower, K., Howson, R., Belle, A., Dephoure, N., O'Shea, E. and Weissman, J. Global Analysis of Protein Expression in Yeast. Nature, 425, 737 - 741 (16 Oct 2003) Letters to Nature .

The use of the TAP-tag for the purification of biomolecule /protein complexes is covered by granted patents in Europe and Australia (EP1105508B1, AU07629621) and patent applications in Canada, Japan and US (see int. pat. appl . WO-0009716).

For licensing information for use in purification of biomolecule /protein complexes, please contact Cellzome AG, Meyerhofstr . 1, 69117 Heidelberg , Germany at info@cellzome.com.

For commercial licensing information regarding further fields of use, please contact Technology Officer, Open Biosystems, Inc., 601 Genome Way, Suite 2100, Huntsville, AL 35806.  Phone: 256-704-4848  Fax :  256-704-4849.

Thermo Fisher Scientific Inc.
Learn more about Thermo Scientific Life Science Research Products

Cell Culture PCR Reagents & Plastics Protein Research High Content Screening
Biopolymers Nucleic Acid Technologies RNAi Technologies

2009 Thermo Fisher Scientific, Inc. and its subsidiaries. All rights reserved