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Home |  Gene Expression | Yeast | Yeast Knockout Strains



Strain
Background
Genotype
Mat A
BY4730
MATa leu2Δ0 met15Δ0 ura3Δ0
Mat Alpha
BY4739
MATalpha leu2Δ0 lys2Δ0 ura3Δ0
Mat A
BY4741
MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
Mat Alpha
BY4742
MATalpha his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0
Het/Hom Dip
BY4743
4741/4742
Homozygous diploids are in the BY4743 background unless 4730/4739 is indicated
Purchasing Information
Cat # Description Literature ACA COM Buy
YSC1053
 Yeast MATa Collection   $2850 $4275
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YSC4298 Yeast MATa (plates 72-73)   $400 $600
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YSC4341
 Yeast MATa (plate 73)   $200 $300
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YSC4506 Yeast MATa (plates 74-75)   $400 $600
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YSC1054
 Yeast MATalpha Collection   $2850 $4275
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YSC4299 Yeast MATalpha (plates 172-173)   $400 $600
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YSC4507
 Yeast MATalpha (plates 174-175)   $400 $600
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YSC1055 Yeast Heterozygous Collection   $3600 $5400
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YSC4300
 Yeast Heterozygous (plates 281-282)   $400 $600
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YSC4343 Yeast Heterozygous Plate 282   $200 $300
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YSC4508
 Yeast Heterozygous (plates 283-284)   $400 $600
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YSC4619 Yeast Heterozygous (plates 285-286)   $400 $600
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YSC1056
 Yeast Homozygous Diploid Collection   $3000 $4500
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YSC4301 Yeast Homozygous (plates 69-70)   $400 $600
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YSC4344
 Yeast Homozygous Plate 70   $200 $300
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YSC4509 Yeast Homozygous (plates 71-72)   $400 $600
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YSC1057
 Yeast Essential Collection   $655 $975
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YSC1061 Yeast Parental Strain BY4730   $50 $50
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YSC1062
 Yeast Parental Strain BY4739   $50 $50
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YSC1048 Yeast Parental Strain - BY4741   $50 $50
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YSC1049
 Yeast Parental Strain - BY4742   $50 $50
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YSC1050 Yeast Parental Strain - BY4743   $50 $50
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YSC1060
 S288C   $50 $50
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YSC1021 Yeast: Yeast Knock Out Strain   $50 $50 Click to show search field
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YSC1071
 Yeast W303 Heterozygous Diploid Strain   $50 $50
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YSC1058 Yeast W303 MATa Strain   $50 $50
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YSC1059
 Yeast W303 MATalpha Strain   $50 $50
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Yeast Knockout Strains
The Saccharomyces Genome Deletion Project1 has developed a unique collection of knock-out strains covering 96% of the yeast genome. This collection of over 6,000 gene-disruption mutants* provides a unique tool for the functional analysis of the yeast genome. The inclusion of distinct tags - or "molecular barcodes" - that identify each strain allows phenotypic analysis to be performed on a single gene basis or a genome-wide scale2. By using the yeast knock out strains for functional profiling, one can also gather much information about human gene function by analogy. Open Biosystems brings you access to the Yeast Knock-Out strains (YKO), a valuable resource for understanding gene function.

The yeast knock out strains were systematically created using a PCR-based strategy3. By means of two sequential PCR reactions - the first to incorporate the appropriate tags and confer the antibiotic resistance gene and the second to incorporate the mitotic recombination sites - each ORF was replaced with a KanMX cassette using homologous recombination. This method allowed for greater than 95% of the ORFs to be knocked-out. Each cassette contains a unique 20 base pair nucleotide sequence of DNA known as a "molecular barcode" allowing for parallel analysis. Also incorporated is a common set of flanking DNA tag sequences allowing amplification of the unique tags.

Evidence for the functional role of a gene can be obtained by analysis of the phenotypic changes exhibited by each mutant strain under a given condition. While this type of analysis has been performed in the past using traditional genetic screens or random mutagenesis, the yeast knock out strains offer advantages over both of these methods.

One advantage is that the phenotypes of the yeast mutant strains reflect a complete loss of function of the given gene, where this is not assured using classical methods. Furthermore, in contrast to traditional screening, the gene identification is known a priori, thereby removing the time-consuming task of determining the responsible gene.

Also eliminated is the time and tediousness involved in creating a single yeast knock out strain. A single knock out strain is already available for nearly all of the yeast genes in the genome, so all you have to do is request the strain of interest.

The mutant yeast strains can also be analyzed individually via sequencing or by using a whole genome approach. The molecular barcodes allow for parallel analysis of gene function using oligonucleotide arrays. The fitness of a given strain under a specific condition can be determined by calculating the abundance of that strain's DNA. This is achieved using an oligonucleotide array printed with the complementary tag sequence necessary to detect the presence of a molecular barcode1,2.

A wealth of information - including genetic and physical maps along with DNA sequence information - is available from the Saccharomyces Genome Database (SGD) at Stanford University.

* While >6000 represents the numb