Open Biosystems - Yeast Knockout Strains

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Home | Gene Expression | Yeast | Yeast Knockout Strains

Strain	Background	Genotype
Mat A	BY4730	MATa leu2Δ0 met15Δ0 ura3Δ0
Mat Alpha	BY4739	MATalpha leu2Δ0 lys2Δ0 ura3Δ0
Mat A	BY4741	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
Mat Alpha	BY4742	MATalpha his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0
Het/Hom Dip	BY4743	4741/4742
Homozygous diploids are in the BY4743 background unless 4730/4739 is indicated

Purchasing Information
Cat #	Description	Literature	ACA	COM	Buy
YSC1053	Yeast MATa Collection		$2,995.00	$4,490.00
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YSC4298	Yeast MATa (plates 72-73)		$420.00	$630.00
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YSC4341	Yeast MATa (plate 73)		$210.00	$315.00
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YSC4506	Yeast MATa (plates 74-75)		$420.00	$630.00
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YSC1054	Yeast MATalpha Collection		$2,995.00	$4,490.00
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YSC4299	Yeast MATalpha (plates 172-173)		$420.00	$630.00
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YSC4507	Yeast MATalpha (plates 174-175)		$420.00	$630.00
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YSC1055	Yeast Heterozygous Collection		$3,780.00	$5,670.00
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YSC4300	Yeast Heterozygous (plates 281-282)		$420.00	$630.00
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YSC4343	Yeast Heterozygous Plate 282		$210.00	$315.00
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YSC4508	Yeast Heterozygous (plates 283-284)		$420.00	$630.00
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YSC4619	Yeast Heterozygous (plates 285-286)		$420.00	$630.00
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YSC1056	Yeast Homozygous Diploid Collection		$3,150.00	$4,725.00
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YSC4301	Yeast Homozygous (plates 69-70)		$420.00	$630.00
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YSC4344	Yeast Homozygous Plate 70		$210.00	$315.00
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YSC4509	Yeast Homozygous (plates 71-72)		$420.00	$630.00
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YSC1057	Yeast Essential Collection		$690.00	$1,025.00
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YSC1061	Yeast Parental Strain BY4730		$55.00	$55.00
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YSC1062	Yeast Parental Strain BY4739		$55.00	$55.00
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YSC1048	Yeast Parental Strain - BY4741		$55.00	$55.00
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YSC1049	Yeast Parental Strain - BY4742		$55.00	$55.00
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YSC1050	Yeast Parental Strain - BY4743		$55.00	$55.00
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YSC1060	S288C		$55.00	$55.00
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YSC1021	Yeast: Yeast Knock Out Strain		$55.00	$55.00
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YSC1071	Yeast W303 Heterozygous Diploid Strain		$55.00	$55.00
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YSC1058	Yeast W303 MATa Strain		$55.00	$55.00
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YSC1059	Yeast W303 MATalpha Strain		$55.00	$55.00
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Yeast Knockout Strains

The Saccharomyces Genome Deletion Project¹ has developed a unique collection of knock-out strains covering 96% of the yeast genome. This collection of over 6,000 gene-disruption mutants* provides a unique tool for the functional analysis of the yeast genome. The inclusion of distinct tags - or "molecular barcodes" - that identify each strain allows phenotypic analysis to be performed on a single gene basis or a genome-wide scale². By using the yeast knock out strains for functional profiling, one can also gather much information about human gene function by analogy. Open Biosystems brings you access to the Yeast Knock-Out strains (YKO), a valuable resource for understanding gene function.

The yeast knock out strains were systematically created using a PCR-based strategy³. By means of two sequential PCR reactions - the first to incorporate the appropriate tags and confer the antibiotic resistance gene and the second to incorporate the mitotic recombination sites - each ORF was replaced with a KanMX cassette using homologous recombination. This method allowed for greater than 95% of the ORFs to be knocked-out. Each cassette contains a unique 20 base pair nucleotide sequence of DNA known as a "molecular barcode" allowing for parallel analysis. Also incorporated is a common set of flanking DNA tag sequences allowing amplification of the unique tags.

Evidence for the functional role of a gene can be obtained by analysis of the phenotypic changes exhibited by each mutant strain under a given condition. While this type of analysis has been performed in the past using traditional genetic screens or random mutagenesis, the yeast knock out strains offer advantages over both of these methods.

One advantage is that the phenotypes of the yeast mutant strains reflect a complete loss of function of the given gene, where this is not assured using classical methods. Furthermore, in contrast to traditional screening, the gene identification is known a priori, thereby removing the time-consuming task of determining the responsible gene.

Also eliminated is the time and tediousness involved in creating a single yeast knock out strain. A single knock out strain is already available for nearly all of the yeast genes in the genome, so all you have to do is request the strain of interest.

The mutant yeast strains can also be analyzed individually via sequencing or by using a whole genome approach. The molecular barcodes allow for parallel analysis of gene function using oligonucleotide arrays. The fitness of a given strain under a specific condition can be determined by calculating the abundance of that strain's DNA. This is achieved using an oligonucleotide array printed with the complementary tag sequence necessary to detect the presence of a molecular barcode^1,2.

A wealth of information - including genetic and physical maps along with DNA sequence information - is available from the Saccharomyces Genome Database (SGD) at Stanford University.

* While >6000 represents the number of individual genes, there are four backgrounds (haploids of MATa and MATalpha, heterozygous diploid, and homozygous diploid) with many genes available in more than one background, thereby creating over 20,000 knock out strains.

What is Open Labs?

Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products.

Shipping Information:

Individual yeast knock out strains are provided as a live culture in a 2-ml tube. Each tube contains YPD broth with G418 (200µg/mL) and is supplemented with 15% glycerol. Within 3-4 days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at 4°C for up to one week or -80°C indefinitely.

The Yeast Knock Out collections are available as frozen glycerol stocks in 96-well plates. The glycerol stocks are a culture of the Yeast Knock Out in YPD media and glycerol. These plates ship on dry ice and can be maintained indefinitely at -80°C.

Contact our customer service department at info@openbiosystems.com or call at 888-412-2225 for a quote and estimated shipping time on bulk orders.

References:

¹ Winzeler, E.A., et al., Functional characterization of the Saccharomyces cerevisiae genome by gene deletion and parallel analysis, Science, 285:901-906, 1999. ² Giaever, G., et al., Functional profiling of the Saccharomyces cerevisiae genome, Nature 418: 387-391 (2002)
³ Wach, A., Brachat, A., Poehlmann, R. & Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae, Yeast10, 1793-1808.

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