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Purchasing Information
Cat # Description Literature ACA COM Buy
YSC5092
Yeast Cross & Capture System Collection Prey MATalpha   $610.00* $915.00*
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YSC5049 Yeast Cross & Capture System Collection Bait MATa   $610.00* $915.00*
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YSC5095
Yeast Cross & Capture strain - Bait MATa   $55.00* $55.00* Click to show search field
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(only clones from YSC5095)
YSC5096 Yeast Cross & Capture strain - Prey MATalpha   $55.00* $55.00* Click to show search field
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(only clones from YSC5096)
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Yeast Cross and Capture System
Simply and reliably study protein-protein interactions

Dr. Bernhard Suter in the Stagljar Lab at the University of Toronto has created a system to examine protein-protein interactions and protein modifications using 6xHIS and VSV tagged haploid yeast strains.  In the Yeast Cross and Capture System a “bait” MATa strain with a specific gene tagged with 6xHIS is mated with a “prey” MATalpha with a specific gene tagged with VSV to generate a diploid organism.1  The tagged protein and any interacting molecules can then be pulled down via the 6xHIS tag and the VSV can be detected on a western blot with an anti-VSV antibody.

  • Over 200,000 crosses possible
  • Amenable to high-throughput screens
  • Reliable results

Please Note:
We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control. Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. Thermo Fisher Scientific is not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.
Shipping Information:

Individual clones:

Within 2–3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C. Clones are provided as a live culture in a 2-ml tube. Each tube contains YPD + G418 (200ug/ml) + 15% glycerol.

High volume orders:

Orders of greater than 50 clones will be provided in microtiter plates. These will ship on dry ice via overnight delivery and should be stored at –80°C immediately upon receipt.

Complete collection:

Orders for the entire collection are provided in 96-well microtiter plates. These will ship on dry ice via overnight delivery and should be stored at –80°C immediately upon receipt.

References:

1. Suter, B., et al. (2007). Examining protein–protein interactions using endogenously tagged yeast arrays: The Cross-and-Capture system. Genome Res. 17, 1774-1782.


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