Dr. Bernhard Suter in the Stagljar Lab at the University of Toronto has created a system to examine protein-protein interactions and protein modifications using 6xHIS and VSV tagged haploid yeast strains. In the Yeast Cross and Capture System a “bait” MATa strain with a specific gene tagged with 6xHIS is mated with a “prey” MATalpha with a specific gene tagged with VSV to generate a diploid organism.1 The tagged protein and any interacting molecules can then be pulled down via the 6xHIS tag and the VSV can be detected on a western blot with an anti-VSV antibody.
- Over 200,000 crosses possible
- Amenable to high-throughput screens
- Reliable results
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Individual clones:
Within 2–3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C. Clones are provided as a live culture in a 2-ml tube. Each tube contains YPD + G418 (200ug/ml) + 15% glycerol.
High volume orders:
Orders of greater than 50 clones will be provided in microtiter plates. These will ship on dry ice via overnight delivery and should be stored at –80°C immediately upon receipt.
Complete collection:
Orders for the entire collection are provided in 96-well microtiter plates. These will ship on dry ice via overnight delivery and should be stored at –80°C immediately upon receipt.
1. Suter, B., et al. (2007). Examining protein–protein interactions using endogenously tagged yeast arrays: The Cross-and-Capture system. Genome Res. 17, 1774-1782.
