Developed by Dr. Corey Nislow and Dr. Guri Giaever¹ at the University of Toronto, the Yeast Barcoder Collection is a set of approximately 1400 donor strains with unique DNA sequence tags (barcodes) that can be systematically transferred to any S. cerevisiae collection.  Barcodes were transformed into S. cerevisiae strain BY4741, replacing the phenotypically neutral HO locus with two unique bar codes flanking the KanMX4 cassette (Fig. 1).

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