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Cross and select to barcode almost any yeast collection
Yeast Barcode
Developed by Dr. Corey Nislow (University of Toronto), the Barcoder collection provides an efficient way to introduce unique barcodes in to arrayed yeast libraries. Highly parallel bioassays are a cost effective way to perform genome-scale screens. However, inserting these into a current collection can be expensive and time consuming. This collection allows barcodes to be introduced systematically simply by crossing mate-types. The method has been validated in combination with the DaMP collection (Yan et al., 2008).
Purchasing Information
Cat # Description Literature ACA COM Buy
YSC5117
Yeast Barcoder Collection MATa   $1,050.00* $1,315.00* Click to show search field
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Yeast Barcoder Collection
barcode any existing yeast collection

Developed by Dr. Corey Nislow and Dr. Guri Giaever1 at the University of Toronto, the Yeast Barcoder Collection is a set of approximately 1400 donor strains with unique DNA sequence tags (barcodes) that can be systematically transferred to any S. cerevisiae collection.  Barcodes were transformed into S. cerevisiae strain BY4741, replacing the phenotypically neutral HO locus with two unique bar codes flanking the KanMX4 cassette (Fig. 1).


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Open Biosystems partners with leading academic laboratories to make clone resources that they develop available at a reasonable price. Many of these resources address the needs of specialized research communities not served by other vendors. To make this possible, we distribute these resources “as is” and without independently verifying the accuracy of data or performing QC. While errors by our source laboratories are generally infrequent, they inevitably occur and are not the responsibility of Open Biosystems. Click here for more information about Open Labs Products.
Shipping Information:
Yeast barcoders are provided as a live yeast cultures in 96-well format and shipped on dry ice.  Strains are shipped in YPD broth with G418 (300µg/ml) and is supplemented with 15% glycerol. Store the collection indefinitely at -80°C.


References:

Yan, Z., et al, Yeast Barcoders: a chemogenic application of a universal donor-strain collection carrying bar-code identifiers.  Nature Methods, 2008 Aug, 5(8):719-25.



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