elucidate nucleosome functionality
Nucleosome structural integrity underlies the regulation of DNA metabolism and transcription. A versatile library of 486 systematic histone H3 and H4 substitution and deletion mutants was generated in Saccharomyces cerevisiae to investigate the contribution of each amino acid to nucleosome function. Each amino acid residue was systematically substituted with alanine, and all alanine residues were changed to serine.
In addition, each mutant was linked to two unique 20bp barcode sequences, allowing for microarray-based analysis of the library for quantitative or phenotypic assays.
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Dai, J., et al., (2008) "Probing nucleosome function: a highly versatile library of synthetic histone H3 and H4 mutants" Cell, Vol 134(6):1066-1078.
