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Yeast YFP Fusion Strain Cloning Strategy
By the approach employed here, each amplified PCR product was cloned into the donor vector pDONR221, generating an “entry” clone. A subset of the promoter-gene cassettes were subsequently introduced into a destination vector, generating an “expression” clone by the LR reaction indicated. The LR reaction is technically simpler than the initial cloning process; accordingly, the entry clone collection represents a useful resource for recombination-based subcloning, even without extensive experience in Gateway-based techniques
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YSC5108
Yeast YFP Fusion Kinase Collection   $300.00* $375.00*
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Yeast YFP Fusion Collection
localize your protein of interest

The Yeast Kinase YFP Fusion collection, developed by Anuj Kumar’s research laboratory at the University of Michigan, is a toolkit for a variety of systematic and large-scale localization studies exploring pathway biology in the budding yeast.  Approximately 120 kinase genes have been cloned with a C-terminal YFP tag and 1kb upstream sequence (to encompass native promoters) into a Gateway® donor vector.

Gateway is a registered trademark of Invitrogen Corp.

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