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Yeast YFP Fusion Strain Cloning Strategy
By the approach employed here, each amplified PCR product was cloned into the donor vector pDONR221, generating an “entry” clone. A subset of the promoter-gene cassettes were subsequently introduced into a destination vector, generating an “expression” clone by the LR reaction indicated. The LR reaction is technically simpler than the initial cloning process; accordingly, the entry clone collection represents a useful resource for recombination-based subcloning, even without extensive experience in Gateway-based techniques
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Cat # Description Literature ACA COM Buy
YSC5108
 Yeast YFP Fusion Kinase Collection   $300.00* $375.00*
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Yeast YFP Fusion Collection
localize your protein of interest

The Yeast Kinase YFP Fusion collection, developed by Anuj Kumar’s research laboratory at the University of Michigan, is a toolkit for a variety of systematic and large-scale localization studies exploring pathway biology in the budding yeast.  Approximately 120 kinase genes have been cloned with a C-terminal YFP tag and 1kb upstream sequence (to encompass native promoters) into a Gateway® donor vector.

Gateway is a registered trademark of Invitrogen Corp.

Please Note:
We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control. Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. Thermo Fisher Scientific is not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.
References:
Ma, J., et al. (2008). Localization of autophagy-related proteins in yeast using a versatile plasmid-based resource of fluorescent protein fusions. Autophagy 4(6):792-800.

Bharucha, N., et al. (2008). Analysis of the Yeast Kinome Reveals a Network of Regulated Protein Localization during Filamentous Growth. Mol Biol Cell 19(7):2708-17.