In excess of 90% of Ewing's sarcomas contain a t(11;22) translocation which fuses the EWS gene on chromosome 22 with the FLI1 gene on chromosome 11. This genetic feature defines the Ewing's family of tumors (Ewing's sarcomas, peripheral primitive neuroectodermal tumors (pPNET) and Askin's tumors). In a minority of Ewing's tumors the EWS gene has a different fusion partner including the ERG, ETV1 and the EA1F genes. This disease specific set contains CCAP BACs that span the known breakpoint regions in order to detect disruption of the normal sequence using FISH.

 

Additionally, partial or complete trisomies have been associated with these cancers or with the disease progression. Probes that would allow the detection of these can be selected from the NCI CCAP collection.