Open Biosystems brings
you access to sets of ordered, mapped human BAC clones that cover the
entire human genome. The availability of mapped BAC clones for
chromosomal analysis greatly expedites the identification, localization
and understanding of genes associated with human disease and
chromosomal aberrations.
A genome wide collection of
evenly distributed mapped BAC clones has been made available by Dr.
Vivian Cheung's lab at the University of Pennsylvania1 . Beginning with the RPCI-11 human male BAC library2,
STS markers were used as anchors to map the BACs to the genome, at ~1Mb
resolution. A subset of these clones were used in the NCI CCAP project
and the results of the FISH analyses performed by the NCI CCAP on these
clones are available at both the GenMapDB, and the CCAP web page.
GenMapDB,
a relational database at the University of Pennsylvania, was developed
in order to manage the accumulated data from the BAC mapping project3.
GenMapDB contains the positional location of each BAC clone.
Additionally, the GenMapDB includes restriction fragment analysis and
end sequences for many of the clones. Also included is other useful
information on the clone, markers, and important links to other genome
databases. The data is ordered by chromosomes and you can search either
by specific BAC location, whole chromosome, or physical distance.
To find a BAC clone covering a particular region:
- Point your browser to http://genomics.med.upenn.edu/genmapdb/
- Select 'Start using this database'
- Enter a whole chromosome number or a physical distance in Mb from the p-telomere
- Note the RPCI BAC clone ID and chromosome for placing your order.
Individual clones:
Clones are provided as a live culture in a
2-ml tube. Each tube contains LB medium supplemented with 8% glycerol
and the appropriate antibiotic. Within 4-6 days of receiving your order
we will ship your clone at room temperature via express delivery. Store
the stock clone at -80°C.
Clone Collections:
Orders of 50
clones or greater and stock plates will be provided in 96-well
microtiter plates. These will ship on dry-ice via overnight delivery
and should be stored at -80°C immediately upon receipt. Please contact
our customer service department for a quote and estimated shipping time
on bulk orders.
1 A resource of mapped human bacterial artificial chromosome clones.
Genome Res. 1999 Oct;9(10):989-93.
Cheung VG, Dalrymple HL, Narasimhan S, Watts J, Schuler G, Raap AK, Morley M, Bruzel A.
2 An improved approach for construction of bacterial artificial chromosome libraries.
Genomics. 1998 52: 1-8
Osoegawa, K., P.Y. Woon, B. Zhao, E. Frengen, M. Tateno, J.J. Caatanese, P.J. de Jong.
3 GenMapDB: a database of mapped human BAC clones.
Nucleic Acids Res. 2001 Jan 1;29(1):144-7.
Morley M, Arcaro M, Burdick J, Yonescu R, Reid T, Kirsch IR, Cheung VG.
