for inducible or constitutive expression of targeted genes
The Rudolf Jaenisch laboratory (MIT), a leader in gene targeting technology, has partnered with Open Biosystems to offer two novel kits for engineering single-copy gene targeted mice.
- The Gene Targeting Kit for Inducible Expression (MES4758) enables titratable control of the mutant or wild type targeted gene with doxycycline. This kit includes KH2 mouse ES cells and appropriate vectors.
- The Gene Targeting Kit for Constitutive Expression (MES4759) enables constitutive overexpression of the mutant or wild type targeted gene. This kit includes C2 mouse ES cells and appropriate vectors.
- All ES cells and vectors may be ordered separately.
All kits and kit components are shipped on dry ice. ES cells should be stored in liquid nitrogen. Vectors should be stored at –80°C.
References:
Beard C, et al. (2006). Efficient method to generate single-copy transgenic mice. Genesis44,
23–28.
Buchholz F, et al. (1998). Improved properties of FLP recombinase evolved by cycling
mutagenesis. Nature Biotechnology16, 657–662.
Hochedlinger K, et al. (2005). Ectopic expression of Oct-4 blocks Progenitor cell differentiation.
Cell121, 465–477.
Urlinger S, et al. (2000). Exploring the sequence space for tetracycline-dependent
transcriptional activators: novel mutations yield expanded range and sensitivity. Proc.
Natl. Acad. Sci. USA97, 7963–7968.
