Dr. Greg Hannon at Cold Spring Harbor Laboratories (CSHL) has created a library of artificial microRNAs (amiRNAs) in collaboration with Drs. Rob Martienssen and Dick McCombie at CSHL and the Weigel lab at the Max Planck Institute for Developmental Biology. Each of the estimated 22,000 Arabidopsis thaliana genes is targeted with 3 amiRNAs, making this the first genome wide resource for plant RNAi .
Highlights include:
- Whole genome coverage with 3 constructs per target gene available
- Validated mir319a backbone
- 35S promoter for strong, constitutive expression of the amiRNA
- Direct plant transformation possible
- Basta® resistance
- Flexible vector design
See Schwab et al 2006 for details on design and validation of the amiRNAs.
The Arabidopsis thaliana amiRNA are available as individual constructs or a subscription to the whole genome library.
Finding your amiRNA of interest
- Enter gene symbol(s), GenBank accession number(s), clone IDs, or catalog numbers into the gene search in the purchase grid. Results will be returned in a tabbed format. Click on shRNA under the shRNAs/RNAi tab.
- Click on the oligo ID link for additional cloning and sequence information
- Order directly online. Constructs are shipped in 48 hours.
Individual clones are provided as a live culture in a 2-ml tube. Each tube contains LB broth with spectinomycin (100µg/ml) and is supplemented with 8% glycerol. Within 3-4 days of receiving your order we will ship your clones as glycerol stock cultures on wet ice. Store the stock clone at 4°C for up to one week or -80°C indefinitely.
The library is provided as frozen glycerol stocks in 96-well plates. The glycerol stocks are a culture of the amiRNA clone in LB media, spectinomycin (100µg/ml) and 8% glycerol. These plates ship on dry ice and can be maintained indefinitely at -80°C.
