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Abnormal cytokinesis in pbl  RNAi phenotypes


 

Drosophila S2 cells 72 hours after the addition of dsRNA to the pebble gene; note the large polyploid cells (a). CON dsRNA targeting the intron of the white gene did not produce any phenotype (b). DNA-blue, Nuclear envelope-green, Filamentous actin- red
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Cat # Description Literature ACA COM Buy
RDM1189
 Drosophila RNAi Library 1.0   $5775 call
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RDM4220 Drosophila RNAi Library 2.0   $7975 call
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RDM4339
 Drosophila RNAi Library- Release 1.0 and 2.0   call call
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RDM4412 Drosophila RNAi Library-dsRNA 5ugs   call call
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RDM4467
 Drosophila RNAi Library-dsRNA 15ugs   call call
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RDM4414 Drosophila RNAi Library-dsRNA 30ugs   call call
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RDM4429
 Drosophila RNAi Library-dsRNA Test plate   $300 $450
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Drosophila RNAi collections
Whole genome RNAi screening

Expression Arrest™ Drosophila RNAi collections from Open Biosystems target the entire Drosophila genome with RNAi constructs.
These collections are available as: 
    dsDNA template libraries ready for in vitro transcription reactions and  
    Ready-to-go dsRNA constructs for high-throughput RNAi experiments.

Both collections were developed in collaboration with the Pat O'Farrell and Ron Vale laboratories at UCSF.

dsDNA template libraries were created by using gene specific primers to amplify 200-800 bases of exonic sequence for each gene. T7 promoter sequences were added to enable dsRNA synthesis in an IVT reaction. Details

The dsRNA construct library was developed to enable rapid and cost-effective high-throughput RNAi screening in Drosophila. In vitro transcription reactions were done on over 15,000 Drosophila genes to produce this set. Details

A test plate for the Drosophila dsRNA library is also available. This test plate contains 8 genes with well known RNAi phenotypes as well as a non-silencing control. Details
 
Shipping Information:
The Drosophila dsDNA and dsRNA libraries are available in 96-well PCR plates. Plates contain frozen constructs and are shipped on dry ice. Store at –20° C. For long term storage it is recommended to store the dsRNA constructs at -70C. The dsRNA product can be stored at -20C for at least 3 months without any sign of dsRNA degradation.  
 
References:
Foley E, O’Farrell PH (2004) "Functional dissection of an innate immune response by a genome-wide RNAi screen". PLoS Biol 2(8): e203.
Echard A., et al (2004) "Terminal cytokinesis events uncovered after an RNAi screen" Current Biology (14) 1685-1693.

Open Biosystems would like to acknowledge the following people for the development of this resource: Pat O’Farrell, Ron Vale, Greame Davis, Edan Foley, Ben Eaton, Nico Stuurman, and Steve Rogers all of the University of California, San Francisco. Though the aforementioned researchers developed this resource, they are not responsible for the product distributed by Open Biosystems.

 

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