Whole genome RNAi screening
Expression Arrest™ Drosophila RNAi collections from Open Biosystems target the entire Drosophila genome with RNAi constructs.
These collections are available as:
dsDNA template libraries ready for in vitro transcription reactions and
Ready-to-go dsRNA constructs for high-throughput RNAi experiments.
Both collections were developed in collaboration with the Pat O'Farrell and Ron Vale laboratories at UCSF.
dsDNA template libraries were created by using gene specific primers to amplify 200-800 bases of exonic sequence for each gene. T7 promoter sequences were added to enable dsRNA synthesis in an IVT reaction. Details
The dsRNA construct library was developed to enable rapid and cost-effective high-throughput RNAi screening in Drosophila. In vitro transcription reactions were done on over 15,000 Drosophila genes to produce this set. Details
A test plate for the Drosophila dsRNA library is also available. This test plate contains 8 genes with well known RNAi phenotypes as well as a non-silencing control. Details
The Drosophila dsDNA and dsRNA libraries are available in 96-well PCR plates. Plates contain frozen constructs and are shipped on dry ice. Store at –20° C. For long term storage it is recommended to store the dsRNA constructs at -70C. The dsRNA product can be stored at -20C for at least 3 months without any sign of dsRNA degradation.
Foley E, O’Farrell PH (2004) "Functional dissection of an innate immune response by a genome-wide RNAi screen". PLoS Biol 2(8): e203.
Echard A., et al (2004) "Terminal cytokinesis events uncovered after an RNAi screen" Current Biology (14) 1685-1693.
Open Biosystems would like to acknowledge the following people for the development of this resource: Pat O’Farrell, Ron Vale, Greame Davis, Edan Foley, Ben Eaton, Nico Stuurman, and Steve Rogers all of the University of California, San Francisco. Though the aforementioned researchers developed this resource, they are not responsible for the product distributed by Open Biosystems.
