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Visualize transfection complexes

Figure1. Cos-1 cells transfected with plasmid DNA using Fluoescent Arrest-In. Five hours after transfection, fluorescent Arrest-In-DNA complexes are clearly seen surrounding the nuclei of transfected cells.

Purchasing Information
Cat # Description Literature ACA COM Buy
ATR4164
 Fluorescent Arrest-In Transfection Reagent (0.5ml)   $150 $225
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ATR4165 Fluorescent Arrest-In transfection reagent (1.0mL)   $225 $340
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ATR4166
 Fluorescent Arrest-In Transfection Reagent (5x1ml)   $725 $1100
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ATR4167 Fluorescent Arrest-In Transfection Reagent (10x1ml)   $1050 $1575
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Fluorescent Arrest-In
Dual function reagent for shRNA delivery
Fluorescent (Fl) Arrest-In™ is sulforhodamine B-conjugated Arrest-In™. It was developed to enable visualization in real-time, of Arrest-In-DNA complexes in cells. Fl Arrest-In™ is a  dual function reagent, permitting visualization of transfected complexes as well as efficient delivery of shRNA into the nucleus of transfected cells. Features include:
  • Lipo-polymer based formulation optimized for high efficiency delivery of shRNA
  • Rhodamine fluorescent tag enables visualization and tracking of transfection events
  • Perform double label experiments
  • Works effectively on adherent and suspension cell lines
  • Low cytotoxicity  
Double label studies with Green Fluorescent Protein (GFP)
Figure 2. Hela cells stably expressing GFP were transfected with Fl Arrest-In-DNA complexes. Images were generated 5 and 48 hours after transfection. Red: Fluorescent Arrest-In-DNA complexes
Green: GFP expression Blue: Nucleus Yellow: Co-localized Fluorescent Arrest-In and GFP
The rhodamine fluorescent tag (excitation is at 560nm and emission at 580nm) allows double label experiments with a variety of commonly available tags (GFP, FITC, biotin etc.). Fl Arrest-In has been tested on a variety of cell types and has shown transfection efficiencies comparable to that seen using unconjugated Arrest-In™. Studies to assay for cytotoxic effects of both Arrest-In and Fl Arrest-In show that both reagents had minimal to no cytotoxic effects when compared against untreated cells. See product insert for data.
 
Shipping Information:
Fl Arrest-In™ is supplied in sterile filtered water at a concentration of 1 mg/ml. 0.5ml, 1ml, 5x1ml and 10x1ml sizes are available. Store at 4°C for upto 12 months. Do not freeze.
References:
Pollard et al (1998) Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to the Nucleus in Mammalian Cells. Journal of Biological Chemistry, Vol 273: 13; 7507-7511.

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