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Home |  RNAi | MicroRNA-adapted Retroviral Ve

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Cat # Description Literature ACA COM Buy
EAV4678
 TMP microRNA-adapted retroviral vector, glycerol stock   $432.00* $432.00*
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EAV4679 LMP microRNA-adapted retroviral vector, glycerol stock   $432.00* $432.00*
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(only clones from EAV4679)
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MicroRNA-adapted Retroviral Vectors
for flexible shRNAmir cloning and expression
A series of microRNA-adapted retroviral vectors have been recently developed in the laboratory of Scott Lowe at CSHL to enable the efficient expression of shRNAmir constructs from RNA Polymerase II (Pol II) promoters (Dickens et al 2005). These vectors produce highly efficient knockdown even when present at single copy in the genome. Since Pol II transcribes endogenous primary microRNA transcripts, the improved performance of these vectors seems to derive from natural mechanisms of RNA-dependent gene inhibition. Features of the vectors include:
  • Efficient and stable single copy knockdown
  • Inducible expression with Tet-responsive promoters
  • Easy cloning of shRNAmir from pSM2 into Pol II retroviral vectors
  • Validated for in vitro and in vivo use

Tetracycline-regulated retroviral vector- SIN-TREmiR30-PIG(TMP)

  • MSCV-based self-inactivating (SIN)retroviral vector
  • Express shRNAmir from tetracycline-regulated Pol II promoter (TRE-CMV)
  • Regulated gene knockdown in tet-on or tet-off configurations.
  • Select stableintegrants using Puromycin resistance
  • GFP serves as amarker of shRNA expression

P-Prime

MSCV/LTRmiR30-PIG (LMP)

  • MSCV-based retroviral vector 
  • Express shRNAmir from the retroviral LTR promoter
  • Select stable integrants using puromycin resistance
  • GFP serves as a marker for retroviral integration

P-Prime-2


This product is covered by a Limited Use License
Shipping Information:
Each vial of Expression Arrest Pol II retroviral vectors is shipped at a concentration of 0.5µg/µl in a total volume of 20µl, thus providing a total amount of 10µg vector DNA. The vector DNA is shipped in a microfuge tube at room temperature and should be stored at -20°C or -80°C.
References:

Dickins RA et al (2005) "Probing tumor phenotypes using stable and regulated synthetic microRNA precursors" Nature Genetics Vol 37, No 11 1289-1295.


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