microRNAs are endogenous non-coding ~22mer RNAs that are highly conserved in mammals and that regulate gene expression post-transcriptionally through innate RNA interference mechanisms. To study long-term microRNA-induced phenotypes, Thermo Fisher Scientific has developed a completely novel system for stable expression of the mature microRNA (Figure 1).

Thermo Scientific miRIDIAN shMIMIC Lentiviral microRNAs were developed using a unique and proprietary design strategy resulting in highly functional microRNA mimics. miRIDIAN^® shMIMIC^™ Lentiviral microRNA designs are created for the robust over-expression of each mature human microRNA in the miRBase database while minimizing the effects of the star (*) strand. Further, shMIMIC microRNAs are provided as lentiviral particles for delivery into biologically relevant cells and creation of stably integrated, microRNA-expressing cell lines.

Unique, patent-pending expression scaffold for consistent and correct processing of the mature microRNA

• Universal primary context ensures that each mature microRNA is correctly and consistently processed
• Design modifications promote preferential loading of the mature microRNA into RISC and robust function

Lentiviral vector system permits viral particle production and delivery into cells refractory to lipid-based delivery

• Expression of TurboGFP (Evrogen, Moscow) allows visualization of transduction efficiency (Figure 2)
• Generation and maintenance of stable cell lines facilitated by puromycin selection marker
• VSVg envelope protein permits transduction into a broad range of cells
• Integrated over-expression of mature microRNA in biologically relevant cell lines advances long-term study of microRNA-induced gene target modulation and phenotypic effects (Figure 3)

• miRIDIAN shMIMIC microRNA design
• miRIDIAN shMIMIC microRNA formats
• miRIDIAN shMIMIC microRNA brochure

Figure 2: TurboGFP expression allows visual tracking of shMIMIC microRNA TurboGFP expression in four different cell lines (HEK293, HUVEC, K562, SH-SY5Y) visualized by epifluorescence microscopy 120 hours post-transduction with various shMIMIC microRNA (no puromycin selection).

Figure 3: shMIMIC microRNAs induced repression of endogenous human microRNA targets HEK293, HUVEC, K562, or SH-SY5Y cells were transduced in biological triplicate with specific shMIMIC microRNA or negative control viral particles. Cells were harvested for RNA isolation 120 hours post-transduction (no puromycin). shMIMIC microRNA-induced repression of endogenous gene targets was determined using either Thermo Scientific Solaris RT-qPCR gene assays or Thermo Scientific Verso SYBR Green 2-Step QRT-PCR Kit. Data was normalized to a housekeeping gene (PPIB) and further normalized to matched shMIMIC negative control

Enter microRNA ID, accession, symbol, or catalog number into the gene search . Results will be returned in a tabbed format. Click on the microRNA tab to see all shMIMIC microRNA constructs returned for your search criteria. Click on the oligo ID for additional information. miRNA sequence can be obtained by clicking on the accession link to miRBase in the gene search result. Order directly online, or via phone, fax or email.