The RNAi consortium (TRC) is a collaborative effort based at the Broad Institute of MIT and Harvard, and includes six MIT and Harvard associated research institutions and five international life sciences organizations. Over three years TRC will create lentiviral shRNA libraries targeting 15,000 human (TRC-Hs 1.0) and 15,000 mouse (TRC-Mm1.0) annotated genes with a goal of validating each shRNA in multiple cell lines. Open Biosystems has partnered with the TRC to make these shRNA libraries available to researchers worldwide.

Features of the TRC shRNA libraries include:

• Rules-based shRNA design for efficient gene knockdown
• Already cloned into lentiviral vectors
• Amenable to in vitro and in vivo applications such as the creation of stable knockdowns
• Lentiviral vector enables transduction of primary and non-dividing cell lines
• Broad coverage: 4-5 constructs per gene

Click for more TRC shRNA Library details

Click here for GO annotated gene families and pathways

Finding your shRNA of interest

1. Enter gene symbol(s), GenBank accession number(s), clone IDS, Unigene clusters or catalog numbers into the gene search or  the shRNA search in the purchase grid.
2. Results will be returned in a tabbed format. Click on shRNAs under the shRNAs/RNAi tab.
3. Click on the oligo ID link for additional information including hairpin sequences.
4. Order directly online. Constructs are shipped in 48 hours.

Alternatively you can send your gene symbols or accession numbers to us atinfo@openbiosystems.comand our technical representatives will perform your search for you.

Citing the library in Scientific Publications

In scientific publications, the libraries should be referred to as TRC-Hs1.0 (Human) and TRC-Mm1.0 (Mouse). Individual clones are uniquely identified by their TRC ID number (eg. TRCN0000014783).
Further information is available at http://www.broad.mit.edu/genome_bio/trc/rnai.html

This product is covered by a Limited Use License.