Decode™ RNAi viral screening pools combine the advantages of shRNAmir design, the power of viral delivery and the convenience of ready-to-use pooled viral particles to facilitate multiplexed RNAi screening. The high titer viral pool format enables genome-scale RNAi screening without the cost and labor associated with individually arrayed screens. Perform genome-scale RNAi screens for genes regulating cellular responses, signaling pathways or discover genes with novel functions - all at the convenience of your benchtop.
The genome-wide human GIPZ lentiviral shRNAmir library is now available in Decode RNAi viral screening pool format and has the following advantages:
- GIPZ Lentiviral shRNAmir produces efficient single copy knockdown
- High titer viral pool format facilitates multiplexed RNAi screens
- Lentiviral delivery extends RNAi screening to primary and non-dividing cells
- Primers for identification of hits are included
Decode RNAi viral screening pools are available in a kit optimized for positive selection screening which includes:
- Seven viral pools each containing 10K GIPZ lentiviral shRNAmir
- Non-silencing control shRNAmir construct-Lentiviral particles
- PCR primers for shRNAmir amplification
- Positive control plasmid DNA for PCR amplification
- Sequencing primer to identify shRNAmir
Decode RNAi viral screening pools are provided as frozen viral particles containing at least 5x10^8 transducing units per ml in DMEM (Dulbecco's Modified Eagle's Medium) with no serum or antibiotics and are ready to use in RNAi experiments. Pooled virus is shipped on dry-ice and should be stored in a -80ºC freezer upon arrival. Decode RNAi screening pools will remain stable for at least one year without any appreciable loss of titer. The vials shipped in the kit have not been thawed, and therefore the indicated titers are accurate for the first thaw. The certificate of analysis contains lot-specific titer and production dates for controls.
