• shRNAmir design for specificity and increased knockdown efficiency
• TurboGFP marks cells expressing shRNAmir
• Efficient low copy knockdown- Important for pooled screens
• Genome-wide human and mouse coverage
• Perform efficient RNAi in primary and non-dividing cells
• Guaranteed* knockdown

Please note that the pGIPZ and pTRIPZ vectors are not compatible with third generation packaging systems such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.

Transduction efficiency of pGIPZ TurboGFP expression from the pGIPZ vector allows measurment of transfection and transduction efficiency. It also effectively marks shRNAmir expression in target cells. A. HEK293 cells 48 hrs post-transduction and B. the corrosponding phase image. 100% transduction efficiency is seen in these cells

Knockdown efficiency of GIPZ lentiviral shRNAmir HEK293T cells were transduced with lentiviral particles containing shRNAmir to GAPDH, eG5, p53, Rb or STAT6. non-silencing shRNAmir was transduced as a control. The graph depicts the residual levels of these genes normalized to their equivalent non-silencing control

1. Enter gene symbol(s), GenBank accession number(s), clone IDs, unigene clusters or catalog numbers into the gene search or into the shRNA search () in the purchase grid. Results will be returned in a tabbed format. Click on shRNAs under the shRNAs/RNAi tab.
2. Target gene sets will be shown for all targets that have greater than two shRNAmir/target. Click on the oligo ID link for additional information including hairpin sequences.
3. Order directly online. Constructs are shipped in 48 hours.