- shRNAmir design for specificity and increased knockdown efficiency
- TurboGFP marks cells expressing shRNAmir
- Efficient low copy knockdown- Important for pooled screens
- Genome-wide human and mouse coverage
- Perform efficient RNAi in primary and non-dividing cells
- Guaranteed* knockdown
Please note that the pGIPZ and pTRIPZ vectors are not compatible with third generation packaging systems such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.
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TurboGFP expression from the pGIPZ vector allows measurment of transfection and transduction efficiency. It also effectively marks shRNAmir expression in target cells. A. HEK293 cells 48 hrs post-transduction and B. the corrosponding phase image. 100% transduction efficiency is seen in these cells |
Knockdown efficiency of GIPZ lentiviral shRNAmir
HEK293T cells were transduced with lentiviral particles containing shRNAmir to GAPDH, eG5, p53, Rb or STAT6. non-silencing shRNAmir was transduced as a control. The graph depicts the residual levels of these genes normalized to their equivalent non-silencing control |
The GIPZ lentiviral shRNAmir library can be purchased as individual human or mouse constructs, target gene sets, RNAintro starter kits or entire libraries. Validated GIPZ controls- positive and negative controls are available in glycerol stock or viral particle formats. *When used in GIPZ RNAintro kits according to kit instructions.
Additional GIPZ sets include:
Validated human GIPZ cancer set
GO annotated human GIPZ gene families
GO annotated mouse GIPZ gene families Human GIPZ annotated library
Finding your shRNAmir of interest
- Enter gene symbol(s), GenBank accession number(s), clone IDs, unigene clusters or catalog numbers into the gene search or into the shRNA search (
) in the purchase grid. Results will be returned in a tabbed format. Click on shRNAs under the shRNAs/RNAi tab.
- Target gene sets will be shown for all targets that have greater than two shRNAmir/target. Click on the oligo ID link for additional information including hairpin sequences.
- Order directly online. Constructs are shipped in 48 hours.
Alternatively you can send your gene symbols or accession numbers to us at info@openbiosystems.com and our technical representatives will perform your search for you.This product is covered by the following limited use licenses:
Limited Use License-shRNA technologies
Limited Use License-ddRNAi
TurboGFP and TurboRFP are covered by a Limited Use License from Evrogen.