- shRNAmir design for specificity and increased knockdown efficiency
- TurboGFP marks cells expressing shRNAmir
- Efficient low copy knockdown- Important for pooled screens
- Genome-wide human and mouse coverage
- Perform efficient RNAi in primary and non-dividing cells
- Guaranteed* knockdown
Please note that the pGIPZ and pTRIPZ vectors are not compatible with third generation packaging systems such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.
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TurboGFP expression from the pGIPZ vector allows measurment of transfection and transduction efficiency. It also effectively marks shRNAmir expression in target cells. A. HEK293 cells 48 hrs post-transduction and B. the corrosponding phase image. 100% transduction efficiency is seen in these cells |
Knockdown efficiency of GIPZ lentiviral shRNAmir
HEK293T cells were transduced with lentiviral particles containing shRNAmir to GAPDH, eG5, p53, Rb or STAT6. non-silencing shRNAmir was transduced as a control. The graph depicts the residual levels of these genes normalized to their equivalent non-silencing control |
The GIPZ lentiviral shRNAmir library can be purchased as individual
human or
mouse constructs, target gene sets,
RNAintro starter kits or entire libraries.
Validated GIPZ controls- positive and negative controls are available in glycerol stock or viral particle formats.
New! High titer lentiviral particles are now available for GIPZ individual constructs Industry-leading high-titer purified virus is now offered at 1x108 TU/ml in 2x25 ul aliquots. This allows for multiple experiments in a variety of cell types requiring variable multiplicity of infection (MOI) and minimizes toxicity. Transduction-ready high-titer lentiviral particle format reduces time, labor and costs involved in prepping DNA, packaging and purifying lentivirus for your RNAi experiments. Purchase 3 constructs per target gene and SAVE over 50%, receive FREE non-silencing and GAPDH positive controls
AND guaranteed** knockdown |
Additional GIPZ lentiviral shRNAmir sub-libraries include:
Validated human GIPZ cancer set
GO annotated human GIPZ gene families
GO annotated mouse GIPZ gene families
Human GIPZ annotated library*When used in GIPZ RNAintro kits according to kit instructions.
**One out of three constructs in lentiviral particle format is guaranteed to knockdown at greater than 70% at the mRNA level. Knockdown should be compared to non-silencing control and experimental workflow controlled using the GAPDH positive control (provided).
Finding your shRNAmir of interest
- Enter gene symbol(s), GenBank accession number(s), clone IDs, unigene clusters or catalog numbers into the gene search or into the shRNA search (
) in the purchase grid. Results will be returned in a tabbed format. Click on shRNAs under the shRNAs/RNAi tab.
- Target gene sets will be shown for all targets that have greater than two shRNAmir/target. Click on the oligo ID link for additional information including hairpin sequences.
- To order viral formats click on the shRNA custom viral production tab and select your construct/s of interest. Select 3 constructs per target gene to save over 50%, get free controls and get guaranteed** knockdown.
- Order directly online. Constructs are shipped in 48 hours.
Alternatively you can send your gene symbols or accession numbers to us at info@openbiosystems.com and our technical representatives will perform your search for you. This product is covered by the following limited use licenses:
