The shRNAmir annotated sub-libraries are derived from GIPZ and pSM2 genome-wide libraries and target only protein-coding genes based on the NCBI's Refseq database. These genes have the most evidence supporting gene function and a more stable annotation than predicted genes having an XM prefix Refseq accessions. The human annotated sub-library has been further subdivided into a hand-curated sub-library that includes shRNAmir that only target genes with clear annotation and significant support in the literature. These focused sub-libraries provide the advantages of shRNAmir design with powerful viral delivery for targeting highly characterized genes in the human and mouse genomes. The broad range of gene ontology covered by these sets make them ideal for uncovering novel pathways or functions.
Annotated genes focused sub-libraries have been created from the pSM2 retroviral libraries and the pGIPZ lentiviral libraries.
Download the individual product data sheets and data files in the purchase grid for details.
| Human | pGIPZ | pSM2 |
| Genes | 18,076 | 18,718 |
| Constructs | 44,602 | 50,339 |
| Mouse | pGIPZ | pSM2 |
| Genes | 18,699 | 19,008 |
| Constructs | 33,851 | 36,720 |
GIPZ lentiviral shRNAmir gene families and pathway rearrays are provided in 96-well microtiter plates containing frozen stock cultures of E.coli in LB (low salt) broth with 8% glycerol, carbenicillin (100ug/ml) and zeocin (25ug/ml).
pSM2 retroviral shRNAmir gene families and pathway rearrays are provided in 96-well microtiter plates containing frozen stock cultures of E.coli in (DH10bpir116) in 2XLB broth with 8% glycerol, chloramphenicol (25ug/ml), and kanamycin (25ug/ml).
Open Biosystems checks all cultures for growth prior to shipment. Store at -80ºC.
