TRIPZ lentiviral shRNAmir, developed in collaboration with Greg Hannon (CSHL) and Steve Elledge (Harvard), combines the advantages of shRNAmir design, Tet-On® inducibility and powerful lentiviral delivery to produce an RNAi trigger that allows tightly regulatable RNAi in most cell types.
Specific features include:
- shRNAmir design produces increased specificity and knockdown
- TurboRFP marks inducible shRNAmir expression
- Inducible knockdown with virtually no leak
- Tet-On® or Tet-Off® configurations possible for regulation
- Entire human genome targeted
- Lentiviral delivery expands regulatable RNAi options in primary and non-dividing cells
Please note that the pGIPZ and pTRIPZ vectors are not compatible with third generation packaging systems such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.
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High levels of knockdown achieved after induction with doxycycline:
Knockdown of indicated target genes in HEK293T cells compared to a non-silencing control as determined by QPCR. Each bar represents an experimental duplicate while QPCR was performed triplicate using 18SrRNA as an internal reference. HEK293T cells were transduced at an MOI=0.3, puromycin selected (5µg/ml), and induced with doxycycline (1µg/ml). |
More detailsFinding your TRIPZ shRNAmir of interest- Enter gene symbol(s), GenBank accession number(s), clone IDs, unigene clusters or catalog numbers into the gene search or into the shRNA search (
) in the purchase grid. Results will be returned in a tabbed format. Click on shRNAs under the shRNAs/RNAi tab.
- Target gene sets will be shown for all targets that have greater than two shRNAmir/target. Click on the oligo ID link for additional information including hairpin sequences.
- Order directly online. Constructs are shipped in 48 hours.
Alternatively you can send your gene symbols or accession numbers to us at info@openbiosystems.com and our technical representatives will perform your search for you.
This product is covered by the following limited use licenses:
Limited Use License-shRNA technologies
Limited Use License-Tet Technology
Limited Use License-ddRNAi
TurboRFP is covered by a Limited Use License from Evrogen.
The TRIPZ lentiviral shRNAmir library is provided in 96-well microtiter plates containing frozen stock cultures of E.coli in LB (low salt) broth with 8% glycerol, carbenicillin (100ug/ml) and zeocin (25ug/ml). Individual shRNAmir constructs are shipped as glycerol stock cultures on wet ice. Open Biosystems checks all cultures for growth prior to shipment. Store at -80ºC.
