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Negative Selection Screens

pTRIPZ
Vector Element
Utility
TRETet-inducible promoter
rtTA3Reverse tet-transactivator
UBC promoterDrives rtTA3 expression of rtTA3 and IRES-puro
cPPTCentral Polypurine tract
helps translocation into the nucleus of non-dividing cells
WREEnhances the stability and translation of transcripts
turbo RFPMarker to track inducible shRNAmir expression
PurorMammalian selectable marker
AMPrAmpicillin bacterial selectable marker
5'LTR5' long terminal repeat
pUC oriHigh copy replication and maintenance in e.coli
SIN-LTR3' Self inactivating long terminal repeat
RRERev response element
ZEOrBacterial selectable marker
Purchasing Information
Cat # Description Literature ACA COM Buy
RHS4696
 Human TRIPZ lentiviral inducible shRNAmir individual clone   $205.00 $205.00 Click to show search field
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(only clones from RHS4696)
RHS4743 Non-silencing-TRIPZ lentiviral inducible shRNAmir control-Glycerol stock   $420.00 $420.00
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(only clones from RHS4743)
RHS4744
 GAPDH-TRIPZ lentiviral inducible shRNAmir positive control-Glycerol stock   $420.00 $420.00
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(only clones from RHS4744)
RHS4741 RNAintro TRIPZ inducible lentiviral shRNAmir transfection kit   $885.00 $1,325.00 Click to assemble kit
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(only clones from RHS4741)
RHS4750
 TRIPZ lentiviral inducible empty vector-Glycerol   $420.00 $420.00
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(only clones from RHS4750)
RHS4700 Human TRIPZ lentiviral inducible shRNAmir library-Release 8.1   call call
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(only clones from RHS4700)
RHS4701
 Human TRIPZ lentiviral inducible shRNAmir library-Release 8.2   call call
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(only clones from RHS4701)
RHS4702 Human TRIPZ lentiviral inducible shRNAmir library-Release 8.3   call call
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(only clones from RHS4702)
RHS4703
 Human TRIPZ lentiviral inducible shRNAmir library-Release 8.4   call call
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(only clones from RHS4703)
RHS4704 Human TRIPZ inducible lentiviral shRNAmir library-Release 8.5   call call
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(only clones from RHS4704)
RHS4705
 Human TRIPZ inducible lentiviral shRNAmir library-Release 8.6   call call
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(only clones from RHS4705)
RHS4706 Human TRIPZ inducible lentiviral shRNAmir library-Release 8.7   call call
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(only clones from RHS4706)
RHS4707
 Human TRIPZ inducible lentiviral shRNAmir library-Release 8.8   call call
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(only clones from RHS4707)
RHS4708 Human TRIPZ inducible lentiviral shRNAmir library-Release 8.9   call call
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(only clones from RHS4708)
RHS4697
 Human TRIPZ lentiviral inducible shRNAmir library subscription (Releases 8.1-8.13)   call call
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(only clones from RHS4697)
search:Search add to cart:Cart product kit: Kit product insert:Literature datafile:Data File

Thermo Scientific Open Biosystems
TRIPZ lentiviral inducible shRNAmir
for tightly regulatable RNAi
TRIPZ lentiviral shRNAmir, developed in collaboration with Greg Hannon (CSHL) and Steve Elledge (Harvard), combines the advantages of shRNAmir design, Tet-On® inducibility and powerful lentiviral delivery to produce an RNAi trigger that allows tightly regulatable RNAi in most cell types.

Specific features include:
  • shRNAmir design produces increased specificity and knockdown
  • TurboRFP marks inducible shRNAmir expression
  • Inducible knockdown with virtually no leak
  • Tet-On® or Tet-Off® configurations possible for regulation
  • Entire human genome targeted
  • Lentiviral delivery expands regulatable RNAi options in primary and non-dividing cells

Please note that the pGIPZ and pTRIPZ vectors are not compatible with third generation packaging systems such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.

Knockdown induced by doxycycline
TRIPZ Knockdown
High levels of knockdown achieved after induction with doxycycline:
Knockdown of indicated target genes in HEK293T cells compared to a non-silencing control as determined by QPCR. Each bar represents an experimental duplicate while QPCR was performed triplicate using 18SrRNA as an internal reference. HEK293T cells were transduced at an MOI=0.3, puromycin selected (5µg/ml), and induced with doxycycline (1µg/ml).

More detailsFinding your TRIPZ shRNAmir of interest
  1. Enter gene symbol(s), GenBank accession number(s), clone IDs, unigene clusters or catalog numbers into the gene search or into the shRNA search () in the purchase grid. Results will be returned in a tabbed format. Click on shRNAs under the shRNAs/RNAi tab.
  2. Target gene sets will be shown for all targets that have greater than two shRNAmir/target. Click on the oligo ID link for additional information including hairpin sequences.
  3. Order directly online. Constructs are shipped in 48 hours.
Alternatively you can send your gene symbols or accession numbers to us at info@openbiosystems.com and our technical representatives will perform your search for you.

This product is covered by the following limited use licenses:
Limited Use License-shRNA technologies
Limited Use License-Tet Technology
Limited Use License-ddRNAi
TurboRFP is covered by a Limited Use License from Evrogen.
Shipping Information:
The TRIPZ lentiviral shRNAmir library is provided in 96-well microtiter plates containing frozen stock cultures of E.coli in LB (low salt) broth with 8% glycerol, carbenicillin (100ug/ml) and zeocin (25ug/ml). Individual shRNAmir constructs are shipped as glycerol stock cultures on wet ice. Open Biosystems checks all cultures for growth prior to shipment. Store at -80ºC.

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