- shRNAmir design greatly increases knockdown efficiency
- Viral LTR promoter for increased expression
- Efficient low copy knockdown- Important for pooled screens
- Stable retroviral vector
- Guaranteed* knockdown
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| HEK293T cells were transfected with shRNAmir to GAPDH, Rb or p53. Non-silencing shRNAmir was transfected as a control. The graph depicts the residual levels of these genes normalized to their equivalent non-silencing control. |
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pSMP retroviral shRNAmir can be purchased as individual constructs, target gene sets or a subscription to the entire library. Positive and negative controls are available in glycerol stock format.Finding your shRNAmir of interest- Enter gene symbol(s), GenBank accession number(s), clone IDs, unigene clusters or catalog numbers into the gene search or into the shRNA search (
) in the purchase grid.
- Results will be returned in a tabbed format. Click on shRNAs under the shRNAs/RNAi tab. Click on the oligo ID link for additional cloning and sequence information. Order directly online. Constructs are shipped in 48 hours.
This product is covered by the following limited use licenses:
Limited Use License-shRNA technologies
Limited Use License-ddRNAi
The pSMP retroviral shRNAmir library is provided in 96-well microtiter plates containing frozen stock cultures of E.coli in LB (low salt) broth with 8% glycerol, carbenicillin (100ug/ml). Individual shRNAmir constructs are shipped as glycerol stock cultures on wet ice. Open Biosystems checks all cultures for growth prior to shipment. Store at -80ºC.
