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Low cytotoxicity
Express-In
Figure 2. COS-1 cells were transfected with 0.1 mg complexed DNA at a 10:1 N/P ratio. Cytotoxicity was determined by adding CellTiter 96® reagent to each well after transfection and measuring optical density of the colored product at 490 nm. Cell viability was calculated according to manufacturers instructions.
Purchasing Information
Cat # Description Literature ACA COM Buy
ETR4620
Express-In transfection reagent 0.5ml   $115.00 $165.00
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(only clones from ETR4620)
ETR4621 Express-In transfection reagent 1ml (2x0.5ml)   $135.00 $205.00
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(only clones from ETR4621)
ETR4622
Express-In transfection reagent 5ml (5x1ml)   $560.00 $805.00
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(only clones from ETR4622)
ETR4623 Express-In transfection reagent 10ml (10x1ml)   $785.00 $1,075.00
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(only clones from ETR4623)
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Express-In transfection reagent
for plasmid DNA
  • High transfection efficiency in a variety of adherent and suspension cell lines
  • Low cytoxicity
  • Proprietary lipopolymer promotes nuclear delivery (see reference 1)
High transfection efficiency
Normalized3
Figure 1. Various cell lines were transfected with a luciferase-encoding plasmid using commercially available transfection reagents. Luciferase expression levels (±SD) were normalized to those achieved using Express-In.
Shipping Information:

Express-In™ is supplied in sterile filtered water at a concentration of 1 mg/ml. Store at 4°C for up to 12 months. Do not freeze.

References:

1. Pollard et al (1998) Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to the Nucleus in Mammalian Cells. Journal of Biological Chemistry, Vol 273: 13; 7507-7511.

Trademarks
Express-In is a trademark of Open Biosystems.
Lipofectamine is a trademark of Invitrogen.
Exgene is a trademark of Fermentas.
FuGENE is a trademark of Roche.


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