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Cat # Description Literature ACA COM Buy
CAB4211
 100ug Rabbit Anti-eGFP Antibody   $100 $100
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(only clones from CAB4211)
CAB4169 100ug Rabbit Anti-GST Antibody   $100 $100
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(only clones from CAB4169)
CAB3872
 50ug Rabbit Anti-HA Antibody   $100 $100
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(only clones from CAB3872)
CAB4255 100ug Rabbit Anti-SNAP Antibody   $100 $100
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(only clones from CAB4255)
CAB1001
 50ug Rabbit Anti-TAP Antibody   $220 $220
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(only clones from CAB1001)
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Tag Antibodies
These antibodies can be used in conjunction with anti-rabbit secondary antibodies, such as anti-rabbit HRP (catalog # SAB1003), to readily detect the proteins in assays such as western blotting (standard western blot protocol). Protein A/G or anti-rabbit conjugated agarose beads can be used to pull down the expressed protein after cleavage and dialysis of the lysed cells and probing with the antibody.

Anti-eGFP Antibody1

Enhanced green fluorescent protein (eGFP) is a widely used reporter of gene regulation that gives a stronger whole cell fluorescence signal. The eGFP antibody has been developed by Open Biosystems for the detection of enhanced GFP fusion proteins.

Description:
Protein A/G purified rabbit polyclonal antibody obtained from the immunization with purified recombinant eGFP. The antibody was validated through assay specific applications testing and by electrophoresis confirming purity and identity. 100µg of affinity purified rabbit polyclonal antibody is supplied at 1mg/mL.

Anti-GST Antibody2

Glutathione S-transferase (GST) is an enzyme that catalyzes the conjugation of the glutathione thiol group to electrophilic centers on a suitable substrate. This activity contributes to the detoxification of a wide range of compounds. GST is also commonly used in a gene fusion system to express, purify and detect proteins of interest. The GST antibody has been developed by Open Biosystems for the detection of GST fusion proteins.

Description:
Protein A/G purified rabbit polyclonal antibody obtained from the immunization with purified recombinant GST. The antibody was validated through assay specific applications testing and by electrophoresis confirming purity and identity. 100µg of affinity purified rabbit polyclonal antibody is supplied at 1mg/mL.

Anti-HA Antibody3

The HA tag antibody has been developed by Open Biosystems for the detection of yeast proteins in the HA-tagged yeast collection and yeast ORF fusion collection. Both of these collections contain individual clones expressing single genes with the hemagglutinin epitope tag (HA tag) directly fused. The versatile 19-kDa tandem fusion tag on the yeast ORFs facilitates a range of detection and affinity purification strategies by also incorporating a 6xHis tag and the protein A ZZ domain for reactivity against most mammalian gamma immunoglobins (IgG).

Description:
Affinity purified rabbit polyclonal antibody obtained from the immunization of purified KLH-conjugated peptide representing the published peptide sequence for the HA tag. Serum is collected and antibodies purified by affinity chromatography against the free immunizing peptide linked to sepharose 4B (GE Healthcare). 50µg of affinity purified rabbit polyclonal antibody is supplied at 0.16mg/mL.

Anti-SNAP Antibody4

SNAP is a small molecule that allows for the covalent attachment of a range of probes to a protein of interest. SNAP-tag™ is a trademark of Covalys Biosciences AG. The SNAP antibody has been developed by Open Biosystems for the detection of SNAP-tag fusion proteins.

Description:
Protein A/G purified rabbit polyclonal antibody obtained from the immunization with purified recombinant SNAP. The antibody was validated through assay specific applications testing and by electrophoresis confirming purity and identity. 100µg of affinity purified rabbit polyclonal antibody is supplied at 1mg/mL.

Anti-TAP Antibody5
 
TAP (tandem affinity purification) is a procedure that was developed to purify proteins expressed at their natural level and under native conditions. This strategy allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity or function.  Among other things, the TAP strategy, when combined with mass spectrometry, allows for identification of proteins interacting with any given target protein. The TAP antibody has been developed by Open Biosystems for the detection of TAP yeast fusion proteins but can also be used for the detection of TAP before or after TEV clevage in all cell hosts.

Description:
Affinity purified rabbit polyclonal antibody obtained from the immunization of purified KLH-conjugated peptide representing the c-terminus of the TAP construct after TEV cleavage.  Serum is collected and antibodies purified by affinity chromatography against the free immunizing peptide. 50µg of affinity purified rabbit polyclonal antibody is supplied at 0.5mg/ml.

References:
  1. Crameri, A., et al. Nature Biotechnol.14, 315-319 (1996).
  2. Habig, W.H., et al. J. Biol. Chem.249, 7130-7139 (1974).
  3. Grayhack, E., Dumont, M., et al. Publication pending.
  4. Keppler, A., et al. Nature Biotechnol.21, 86-89 (2003).
  5. Rigaut, G., et al. Nature Biotechnol.17, 1030-1032 (1999).

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