Selective populations of polyclonal antibodies can be isolated from larger total clonal populations to study modified forms of proteins. This includes modifications such as protein cleavage, mutations, polymorphisms, and post-translational modification. A series of different specialized protocols are available to generate reagents to mee these needs. Each type of specialized protocol includes a three-step affinity purification where antibodies to the modified target are enriched and cross-reactive antibodies to the unmodified target are removed through the use of depletion columns. The final purified product is delivered along with quantitative ELISA results that measure the preference of the antibodies to the modified verus unmodified form. Some of the more common modifications we generate antibodies against:
|
phosphorylation | sumoylation | myristolation |
acetylation | polymorphisms | drug binding |
ubiquitination | glycosylation | isoforms |
| splice variants | ligand binding | prenylation |
protein cleavage (neo-epitopes) | mutations | species cross-reactivity |
For a more detailed description of our phosphospecific antibody protocol, click
here.
Our process allows for the production of high-homology antibodies that can distinguish between two closely related isoforms or splice variants, neo-epitope antibodies that can recognize protein cleavage sites, binding specific antibodies that detect proteins only when bound to drug or ligand, fusion protein antibodies (his-tagged, GST, etc.) and much more. For more information on these protocols, see Protocol Deliverables or contact us at info@openbiosystems.com.
For more information on our polyclonal antibody services, please visit Custom Polyclonal Antibodies Frequently Asked QuestionsAntibody Uses and ApplicationsWhy Order from Open Biosystems?Antibody References