The essential role of protein phosphorylation in cell signaling has been well established for many years. The ability to distinguish between different phosphorylation sites within a protein can be quite difficult and complex but is important to understanding cascades and overall protein activity. Open Biosystems specializes in making sites specific antibodies to discrete phosphorylation sites by taking advantage of our proprietary antigen design and conjugation for enhanced antibody performance and utility across assays. Our multi-link chemical conjugation presents phosphopeptides in multiple, alternate conformations not only increasing the titer of antibodies, but also generating a more adaptive population of polyclonal antibodies that are able to recognize epitopes in different conformations as found in different assays.
Open Biosystems phosphospecific antibody protocols includes
- Synthesis and purification of phosphorylated and non-phosphorylated peptides
- Site-specific, mult-link conjugation to KLH or similar carrier
- Immunization into 2-SPF rabbit, 70-day or 90-day protocol
- Serum collection and ELISA titration against phos and non-phos peptides
- Optional affinity purification and depletion against non-phosphorylated peptide
- Semi-quantitative ELISA with purified antibodies against modified and unmodified peptides